甲氧苄啶对蒲公英体外抗菌增效作用的研究  

Study on Synergitic Antibacteria Actions of Trimethoprim Combined with Herba Taraxaci in vitro

作　　者：王新[1] 崔一喆[1] 邵红[1] 韩铁锁[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,大庆163319

出　　处：《中国畜牧兽医》2010年第2期169-171,共3页China Animal Husbandry & Veterinary Medicine

基　　金：黑龙江省农垦总局科技攻关项目(HNKXIV-08-10-02)

摘　　要：采用微量棋盘稀释法测定了蒲公英、甲氧苄啶及两药联用对金黄色葡萄球菌、大肠杆菌和沙门氏菌的最小抑菌浓度(MIC),计算蒲公英与甲氧苄啶联用的组分抑菌浓度(FIC)指数。结果表明,蒲公英与甲氧苄啶联用对金黄色葡萄球菌、大肠杆菌和沙门氏菌的FIC指数分别为0.75、0.75和1,呈相加作用。采用平板计数的方法,测定了蒲公英与甲氧苄啶联用作用于3种细菌1、2、4和8 h后的细菌数,并计算杀菌率。利用最小二乘法对杀菌率进行拟合,计算出甲氧苄啶增强蒲公英体外抗金黄色葡萄球菌、大肠杆菌和沙门氏菌作用的最优添加剂量分别为4.0、1.8和2.0 mg/g。The minimum inhibitory concentrations（MIC） of Herba Taraxaci,trimethoprim and Herba Taraxaci combined with Trimethoprim against Staphylococcus aureus,Escherichia coli and Salmonella were measured individually by the method of microdilution checkerboard techniques.Then the fractional inhibitory concentrations（FIC） indices of Herba Taraxaci combined with Trimethoprim were calculated.The results showed that the FIC values on Staphylococcus aureus,Escherichia coli and Salmonella were respectively 0.75,0.75,1.The effect of their combination taken on additive action.The number of Staphylococcus aureus,Escherichia coli and Salmonella which had been inhabited by Herba Taraxaci combined with Trimethoprim for 1 h,2 h,4 h and 8 h,were enumerated by plate counts methods.Then the killing rate of Herba Taraxaci combined with Trimethoprim were calculated.The experimental data were fitted by least squares method.The optimal dose of Trimethoprim which combined with Herba Taraxaci against Staphylococcus aureus,Escherichia coli and Salmonella in vitro,were respectively 4.0 mg/g,1.8 mg/g and 2.0 mg/g.

关 键 词：蒲公英 甲氧苄啶 抗菌增效作用 

分 类 号：S859.79[农业科学—临床兽医学]