家兔临床型乳腺炎病理模型的制备  

Preparation of Clinical Mastitis Induced by Staphylococcus aureus in Rabbits

作　　者：伍杰[1] 刘娟[1] 程跃红 许春 

机构地区：[1]四川省阿坝州中等职业技术学校,茂县623200 [2]四川省卧龙镇农业服务中心,汶川623006 [3]西藏拉萨市种鸡场,拉萨850000

出　　处：《中国畜牧兽医》2010年第2期223-225,共3页China Animal Husbandry & Veterinary Medicine

摘　　要：为建立家兔乳腺炎模型,本试验将从乳房炎患牛奶样中分离出的金黄色葡萄球菌制成104CFU/mL细菌悬液,并分别注入选取的16只3～4 kg健康泌乳家兔的第3对乳池内(1 mL/乳区),诱发乳腺发炎。观察临床表现,乳腺组织形态学病理变化,并于造模后72 h采集乳腺组织匀浆液进行回归试验。结果显示:①造模后动物出现急性炎症反应,表现为饮食欲降低,甚至废绝;体温、心率和呼吸频率显著升高;造模乳区严重肿胀。②造模乳区的乳腺组织镜检发现有炎性细胞浸润、组织结构破坏等现象。③回归试验全部呈阳性,其生长菌落为金黄色葡萄球菌。结果表明,金黄色葡萄球菌诱发家兔临床型乳腺炎模型与炎症的病理过程和炎性反应一致,可用作病理模型使用。To establish rabbits mastitis model,10^4 CFU/mL/3 to 4 kg（body weight） of Staphylococcus aureus that was isolated from the milk of dairy cattle with mastitis were infused into the third of mammary gland in 16 healthy rabbits.When the clinical mastitis model was challenged,observe its manifestations,pathological changes in breast tissue morphology,and regress the breast tissue fluid after established model 72 hours.The results showed that： ①Post-challenged treated group expressed significant clinical signs,manifested as reduced dietary wishes,and even waste must;body temperature,heart rate and respiratory rate significantly increased;model swollen breast areas.②Challenged district of breast tissue examination found inflammatory cell infiltration,destruction of the organizational structure and so on.③Regression tests were positive by both morphological and biochemical identification was identified as Staphylococcus aureus.The results demonstrated that the rabbits experimental mastitis model infused with Staphylococcus aureus via teat duct was the same as immunopathology and inflammatory response,and can be used as a pathological mode1.

关 键 词：家兔 乳腺炎 病理模型 

分 类 号：S854.4[农业科学—临床兽医学]