三七总皂甙对吗啡戒断大鼠大脑皮质M乙酰胆碱受体mRNA及蛋白表达的影响  

EFFECTS OF PANAX NOTOGINSENG ON M-AChR mRNA AND PROTEIN EXPRESSION IN CORTEX OF MORPHINE WITHDRAWAL RATS

作　　者：马春玲[1] 丛斌[1] 余磊[1,2] 闫玉仙[1,3] 牛增强[1,4] 文迪[1] 倪志宇[1] 李淑瑾[1] 

机构地区：[1]河北医科大学基础医学院法医学系,河北省法医学重点实验室,石家庄050017 [2]中国人民武装警察部队北京总队医院外科,北京100027 [3]中国人民武装警察部队医学院中心实验室,天津300162 [4]山东聊城市人民医院法医鉴定室,聊城252000

出　　处：《中国药物依赖性杂志》2010年第1期20-24,共5页Chinese Journal of Drug Dependence

基　　金：河北省中医药管理局重点资助课题(05053)

摘　　要：目的:研究三七总皂甙(PNS)对吗啡戒断大鼠大脑皮质m2,m5乙酰胆碱受体(AChR)mRNA及蛋白表达的影响,探讨PNS抑制吗啡戒断症状的作用机制。方法:以剂量递增法建立大鼠吗啡依赖模型(MOR组),以腹腔注射(ip)纳洛酮建立催促戒断模型(NAL组),大鼠在给予吗啡的同时,采用3种不同剂量PNS(100、200、400mg.kg-1)灌胃(ig)。采用RT-PCR和Western-blot方法分别观察PNS对吗啡依赖及戒断大鼠皮质m2,m5AChR mRNA及蛋白表达的影响。结果:(1)慢性吗啡作用使皮质m2,m5AChR mRNA及m2AChR蛋白表达明显增加(P<0.01);(2)纳洛酮催促戒断使m2,m5AChR mRNA及蛋白表达进一步升高(P<0.01);(3)PNS可以剂量依赖性地抑制纳洛酮催促戒断所引起的AChR mRNA及蛋白表达的增强。结论:PNS可通过抑制m2,m5AChR mRNA及蛋白的表达缓解吗啡戒断症状。Objective：To investigate the effects of panax notoginseng（PNS）on m2,m5 AChR mRNA and protein expressions in cortex of morphine dependent and naloxone-precipitated withdrawal rats,and to clarify the mechanism by which PNS attenuated morphine withdrawal syndrome.Methods：Models of morphine physical dependent（MOR group）and withdrawal rats（NAL group）were established by subcutaneous injection of morphine in gradually increasing doses and abdominal cavity injection of naloxone,respectively.The rats were treated with PNS by intragastric administration at different doses（100,200,400 mg·kg^-1）simultaneously when administrated with morphine.m2,m5 AChR mRNA and protein expressions were detected by RT-PCR and Western-blot,respectively.Results：（1）Chronic morphine treatment increased m2,m5 AChR mRNA and m2 AChR protein expression in cortex（P〈0.01）.（2）Naloxone increased further m2,m5 AChR mRNA and protein expression in cortex（P〈0.01）.（3）PNS downregulated dose-dependently m2,m5 AChR mRNA and prote in expression induced by naloxone precipitated withdrawal.Conclusion：PNS could alleviate morphine withdrawal symptoms by inhibiting m2,m5 AChR mRNA and protein expression.

关 键 词：三七总皂甙 吗啡戒断综合征 大脑皮质 M乙酰胆碱受体 

分 类 号：R245.319[医药卫生—针灸推拿学] R711.6[医药卫生—中医临床基础]