甲基苯丙胺中毒死亡大白兔体内分布研究  

STUDY ON THE POSTMORTEM DISTRIBUTION OF METHAMPHETAMINE IN POISONED DEATH RABBITS

作　　者：张高勤[1] 王玫[2] 张大明[2] 常红发[3] 刘耀[4] 

机构地区：[1]中国人民公安大学,北京100038 [2]北京市公安局法医检验鉴定中心,北京100085 [3]山西医科大学法医学院,太原030001 [4]公安部物证鉴定中心,北京100038

出　　处：《中国药物依赖性杂志》2010年第1期29-33,共5页Chinese Journal of Drug Dependence

基　　金：公安部重点研究计划项目(2009ZDYJBJSJ001)

摘　　要：目的:建立甲基苯丙胺中毒致死的动物模型,探讨其在致死大白兔体内的分布规律。方法:用6只日本♂大白兔经胃管按照150mg·kg-1(3LD50)给予盐酸甲基苯丙胺建立动物模型,大白兔死后迅速解剖并提取心、肝、脾、肺、肾、脑、肌肉、睾丸、胃、心血、周围血、胆汁、尿液和玻璃体液,用正己烷∶乙醇(20∶1)作为提取溶剂,利用UPLC-MS/MS法检测其中甲基苯丙胺含量,使用SPSS15.0统计软件进行方差分析,采用均数两两比较的SNK法。检验水准为α=0.05。结果:实验大白兔胃内甲基苯丙胺含量最高,其次是脾、肺、肾、睾丸和尿液,而心血、周围血和玻璃体液中含量较低。结论:本实验的结果与相关案例报道基本吻合,这提示胃、脾、肾和尿液是甲基苯丙胺中毒案件鉴定的理想检材,中毒死后的甲基苯丙胺分布规律可为相关案件的鉴定提供一定的依据。Objective：To establish the models of methamphetamine poisoned death models,and investigate the distribution of methamphetamine in poisoned death rabbits.Methods：Six male rabbits were given a dose of 150 mg·kg^-1 weight of methamphetamine by gastric tube at an even speed in three minutes.The heart,lung,liver,spleen,kidney,cerebrum,muscle,spermary,gastric,heart blood,peripheral blood,bile,urine and vitreous were collected and analyzed immediately after the death.These samples were extracted by n-hexane∶ ethanol（v/v,20∶1）.Analysis was performed with a UPLC-MS/MS.The qualitative analysis was based on retention time in the full-scan chromatogram of MS1 coupled with the ion fragmentation spectrum of MS2 in the mass spectrometer.The quantitative analysis was on a working curve method.Results：The concentration detected in gastric was the highest and there was a statistic difference（P〈0.05）.The concentrations detected in spleen,lung,kidney,spermary and urine were also higher.Conclusion：The results were in accord with correlative case reports.Gastric,spleen,kidney and urine may be the ideal samples for forensic identification and the postmortem distribution of methamphetamine may provide some evidence for the investigation of suspected poisoning case.

关 键 词：甲基苯丙胺 死后分布 超高效液相色谱-质谱联用法 

分 类 号：R996[医药卫生—毒理学] R595.4[医药卫生—药学]