经日抗原诱导的肠道粘膜免疫反应——集合淋巴小结作用的形态学研究  

Mucosal Immune Response Through Oral Antigen——Morphologic Study on Function of Peyer's Patch

作　　者：刘文庆[1] 姚丽杰[1] 朱兰芹[2] 

机构地区：[1]齐齐哈尔医学院解剖学教研室 [2]齐齐哈尔医学院卫生所

出　　处：《齐齐哈尔医学院学报》1998年第4期266-269,共4页Journal of Qiqihar Medical University

摘　　要：为探讨粘膜免疫反应的诱导因素,本文采用光镜、电镜和免疫组化法观察了30只BALB/c小鼠经口柯萨奇病毒诱导下集合淋巴小结(Peyer’s Patch PP)中淋巴细胞的类型、超微结构和迁移特点.结果:光镜下PP明显增大,圆顶区(dome区)上皮内淋巴细胞(dome intraepithelial lymphocytes:dIEL)与正常组比较明显增多(P<0.01),而dIEL在分布位置上无显著差异(P>0.05).电镜下胚中心及旁滤泡区细胞增生活跃,出现大量巨噬细胞及树突状细胞淋巴细胞丛.在dome区上皮内发现近绒毛顶的淋巴细胞与下方的淋巴细胞或巨噬细胞有紧密接触.dome区M细胞下方的囊袋内淋巴细胞成群.同时dome区还可见大量内质网丰富淋巴细胞.PP的输出淋巴管和高内皮静脉内充满淋巴细胞,并见有的淋巴细胞正在穿越高内皮,有的正在穿越基膜.结果提示:PP以其抗原传递活跃、淋巴细胞增殖、迁移活跃诱导了经口柯奇病毒产生的粘膜免疫反应,PP的dome区同时发挥了效应功能.In order to study the factors inducing mucosal immune response, the classification, finestructure and distribution of lymphocytes of Peyer's Patch(PP) were observed in 30 BALB/c mices through oral Coxackie virus B2 (CVB2) by means of light microscope, electron microscopy and immuhistochemistry. The results showed that intraepithelium lymphocytes in dome (dIEL) of PP increased obviously ( P <0.01), but there was no significant differnce in dIEL distribution between the experimental group and the control group by light microscopy ( P >0.05). Observed by electron microscope, lymphocytes in germinal center and paraphollicular proliferated greatly, and great number of marcrophage-lymphocytes and dendritic cell-lymphocytes were found. Some dIEL near villi kept in touch with lymphocytes or marcrophages. Lymphocytes in dome area blow M cells gathered in crowds, meanwhile, lymphocytes with plentiful endoplasmic net were found in dome area. PPefferent lymph vessel and high-endothelial venules (HEV) were full of lymphocytes, some were passing through the endothelium or the basement membrane of HEV. The results suggested that PP could induce the mucosal immune response through oral CVB2 with active growth, migration of lymphocytes and presenation of antigen.

关 键 词：柯萨奇病毒B2 集合淋巴小结 淋巴细胞 粘膜免疫 

分 类 号：R392.12[医药卫生—免疫学] R373.23[医药卫生—基础医学]