测定TGF—β1生物活性^3H—TdR细胞生长抑制法和MTT法比较研究  

MEASUREMENT OF BIOACTIVITY OF TGF-β 1 BY CELL GROWTH INHIBITION

作　　者：谌秋华[1] 李勇[1] 韩本立[1] 李昆[1] 

机构地区：[1]第三军医大学附院肝胆外科实验室

出　　处：《九江医学》1998年第3期133-136,共4页Jiujiang Medical Journal

摘　　要：目的：探讨检测转化生长因子－β１（ＴＧＦ－β１）灵敏可靠的方法。方法：用水貂肺上皮细胞（ＣＣＬ－６４）作为抑制细胞，分别采用３Ｈ－ＴｄＲ掺入法和ＭＴＴ法，对照ＴＧＦ－β１标准含量，计算出细胞生长抑制率，比较两法灵敏性和稳定性。结果：ＣＣＬ－６４细胞生长抑制与ＴＧＦ－β１是负相关；回归系数假设检验两种检测方法有显著差异（Ｐ＜００５）；３Ｈ－ＴｄＲ法误差率比ＭＴＴ法小。结论：３Ｈ－ＴｄＲ法测定ＴＧＦ－β１活性较ＭＴＴ法灵敏度高，稳定性好。OBJECTIVE:To determine a sensitive and reliable method measuring human transforming growth factor β 1 (TGF-β 1).METHOD:Using mink lung epithelial cells (CCL-64) as inhibiting cells,TGF-β 1 was measured by 3 H-TdR and MTT.Compared to standard TGF-β 1 contents,cell growth rate was measured and sensitvity and stability were compared between the two methods.RESULTS:CCL-64 and TGF-β 1 were negative correlated.The two methods had significant difference by hypothesis testing on regression coefficient( P <0 05). 3H-TdR had lower error rate than MTT.CONCLUSION: 3H-TdR is more sensitive and stable than MTT in measuring TGF-β 1.

关 键 词：肺上皮细胞 转化生长因子 活性 氢3 TdR法 MTT法 

分 类 号：Q513.03[生物学—生物化学]