4种鼠疫F1抗原/抗体检测方法的初步评价  

Preliminary evaluation of four methods to determine the F1 antigen/antibody of plague

作　　者：王艳华[1] 海荣[1] 夏连续[1] 蔡虹[1] 梁莹[1] 俞东征[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所人兽共患病室,传染病预防控制国家重点实验室,北京102206

出　　处：《中国媒介生物学及控制杂志》2010年第1期39-42,共4页Chinese Journal of Vector Biology and Control

摘　　要：目的客观评价血凝试验、胶体金试验、ELISA和PCR4种检测方法的灵敏性。方法对于血清学方法中的F1抗体检测,评价检出的全菌免疫兔抗血清最低稀释度;F1抗原检测,评价检出的最低浓度。对于分子生物学方法,评价同时检出fra基因和pla基因的模板最低浓度。结果对于F1抗体检测,间接血凝试验检出的最低稀释度是1∶64,胶体金试验是1∶1000,ELISA是1∶204800。对于F1抗原检测,反向血凝试验检出的最低浓度是2ng/ml,胶体金试验是50ng/ml。fra、pla基因同时检出,模板的最低浓度是0.21ng/μl。结论在以抗体IgG为主的血清中,ELISA法对于F1抗体检测的灵敏性较高。反向间接血凝试验对于F1抗原检测的灵敏性较高。为减少误诊率,采用PCR诊断鼠疫菌要求最低模板浓度是0.21ng/μl。Objective To evaluate the sensitivity of the four test methods, hemagglutination test, gold immunochromatographic assay （GICA）, enzyme-linked immune sorbent assay （ELISA） and polymerase chain reaction （PCR）. Methods The minimal detectable dilution of antiserum of a bacteria-immunity rabbit via serological approaches for F1 antibody detection, the minimal detectable concentration in F1 antigen measurement, and the minimal concentration of template with a detectable gene pair,fra and pla, were evaluated. Results For the Fl antibody test, the minimal dilution detected was 1：64 through IHA, 1：1000 through GICA, and 1 ： 204 800 through ELISA. For FI antigen test, the minimal detectable concentration was 2 ng/ml by RIHA and 50 ng/ml by GICA. The minimal concentration of template was 0.21 ng/μl whenfra and pla were both detected. Conclusion In the serum consisting mainly of antibody IgG, the ELISA method has a higher sensitivity detecting F1 antibodies. The RIHA has higher sensitivity detecting FI antigens. To minimize misdiagnosis, a minimal concentration of template of 0.21 ng/μl is required to diagnose plague when using PCR.

关 键 词：鼠疫耶尔森菌 血凝试验 胶体金试验 酶联免疫吸附试验 聚合酶链反应 

分 类 号：R254.8[医药卫生—中医内科学]