给水处理工艺对水中有机提取物致细胞氧化应激的影响  

Cellular Oxidative Stress Induced by Organic Extracts of Drinking Water and the Effect of Drinking Water Treatment Procedures

作　　者：陈艳艳[1] 刘爱林[1] 谢少华[1] 张惠娟[1] 李晓燕[1] 谢虹[1] 鲁文清[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系环境与健康教育部重点实验室,湖北武汉430030

出　　处：《环境与健康杂志》2010年第1期8-10,共3页Journal of Environment and Health

基　　金：国家自然科学基金资助项目(20577012)

摘　　要：目的研究常规给水处理工艺水中有机提取物致细胞氧化应激的影响。方法以人胚肝细胞(L-02细胞)为靶细胞,水样有机提取物设1.2、6、30、150ml/ml培养液四个剂量,二甲基亚砜(DMSO,10ml/L)为溶剂对照,H2O(2100μmol/L)为阳性对照,染毒L-02细胞24h后,检测细胞内脂质过氧化产物丙二醛(MDA)、还原型谷胱甘肽(GSH)和8-羟基脱氧鸟苷(8-OHdG)的含量。结果与溶剂对照相比,给水处理各工艺环节的水样有机提取物在较高浓度(30、150ml/ml培养液)时均使L-02细胞MDA含量增加(P<0.05,P<0.01),GSH含量下降(P<0.05,P<0.01);各工艺环节中MDA含量的变化趋势是:预氯-混凝沉淀工艺使其增加,沉淀工艺使其降低,加氯消毒工艺使MDA含量再次增加;GSH含量在上述相应工艺环节则表现为先下降,再上升,后又下降的趋势。与溶剂对照相比,各工艺环节水样有机提取物浓度为30ml/ml培养液时,L-02细胞中8-OHdG水平升高(P<0.05,P<0.01),且与MDA的变化趋势一致。结论两次加氯工艺增强了有机提取物对L-02细胞的氧化应激作用,使脂质过氧化反应增强、抗氧化作用降低、DNA氧化损伤增加。沉淀、过滤工艺可以减弱上述有害效应。Objective To investigate the effects of drinking water treatment procedures on the cellular oxidative stress induced by organic extracts of drinking water. Methods Human embryonic liver cell line （L-02） was treated with water organic extracts of 1.2, 6, 30 and 150 ml water samples per millililer culture. DMSO （10 ml/L） and H2O2 （100 μmol/L） were used as the negative and positive controls, respectively. MDA, GSH and 8-OHdG in L-02 cell cultures were determined after 24 h of treatment with water organic extracts. Results The contents of MDA were significantly increased and the contents of GSH were significantly decreased in L-02 cells exposed to organic extracts at the concentrations of 30 and 150 ml water samples/ml culture as compared with solvent control （P〈0.05 or P〈0.01）. Changes of MDA level showed tendencies to increase after pre-chlorination plus coagulation-sedimentation and to decrease after precipitation, then to increase again after post-chlorination. The change tendencies of GSH were contrary to those of MDA. Levels of 8-OHdG in L-02 cells exposed to organic extracts at the concentration of 30 ml/ml culture were significantly increased as compared with solvent control （P〈0.05, P〈0.01） and showed the same change tendencies of MDA. Conclusion Both the pre-chlorination and post-chlorination steps lead to the increase of oxidative stress in L-02 cells, but precipitation and filtration show the opposing effects.

关 键 词：水 提取物 脂质过氧化 

分 类 号：R994.6[医药卫生—毒理学]