微囊藻毒素与饮用水有机提取物联合致HepG_2细胞DNA损伤的研究  

Combined Effect of Microcystins and Organic Extracts of Drinking Water on DNA Damage in HepG_2 Cells

作　　者：向伦辉[1,2] 巫丰宏[1] 彭晓琳[1] 谢虹[1] 王家春[1] 鲁文清[1] 吴志刚[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系教育部环境与健康重点实验室,湖北武汉430030 [2]上海市宝山区疾病预防控制中心防疫科,上海201901

出　　处：《环境与健康杂志》2010年第1期11-14,共4页Journal of Environment and Health

基　　金："十一五"国家科技支撑计划项目(2006BAI19B02)

摘　　要：目的探讨微囊藻毒素(MC-LR)与管网末梢水有机提取物联合致HepG2细胞DNA损伤的作用。方法于2007年7月采集某市的管网末梢水,分别选用XAD-2、XAD-8大孔吸附树脂以及两者等体积配制的复合树脂(简称XAD-2/8)富集为100、10和30ml/ml的有机物。应用彗星试验,研究MC-LR与管网末梢水有机提取物联合致HepG2细胞(1×105个/孔)DNA损伤的作用。结果与溶剂对照组相比,0.5μg/mlMC-LR染毒组可使HepG2细胞Olive尾矩值显著增加(P<0.01)。较高浓度(0.1、0.5μg/ml)MC-LR与经XAD-2树脂富集的有机物(100ml/ml)联合染毒时,所诱导的HepG2细胞Olive尾矩值较之相应的单独作用组显著增加(P<0.01);0.05、0.1、0.5μg/mlMC-LR与经XAD-8树脂富集的有机物(10ml/ml)联合染毒时,所诱导的HepG2细胞Olive尾矩值较之相应的单独作用组显著增加(P<0.01);较高浓度(0.1、0.5μg/ml)MC-LR与经XAD-2/8树脂富集的有机物(30ml/ml)联合染毒时,所诱导的HepG2细胞Olive尾矩值较之相应的单独作用组显著增加(P<0.01)。运用析因分析统计学方法,发现MC-LR与3种树脂富集的饮用水有机提取物存在交互作用(P<0.01)。结论饮用水有机提取物与MC-LR之间存在协同作用,MC-LR可以极大地增强饮用水中有机物的致DNA损伤作用。Objective To know the combined effect of microcystins and organic extracts from tap water on DNA damage. Methods Three kinds of macroporous adsorptive resins （XAD-2,XAD-8, XAD-2 and XAD-8 equal proportion mixed resin-XAD- 2/8） were used to concentrate the organic compounds from tap water in Wuhan city in September 2007. The concentrations of organic compounds extracted by XAD-2, XAD-8 and XAD2/8 were 100, 10, 30 ml/ml respectively. The combined effect of MC-LR and organic extracts from tap water on DNA damage in HepG2 cells （1 ×105/hole） was determined by using comet assay. Results The high concentration of MC-LR （0.5 μg/ml） induced significant increase of OTM compared with the negative control （P〈0.01）. When HepG2 cells were co-exposed to MC-LR （0.1 μg/ml,0.5 μg/ml） and organic compounds concentrated by XAD-2 （100 ml/ml）, OTM were significantly increased compared with the corresponding alone exposure（P〈0.01）. When HepG2 cells were co-exposed to MC-LR （0.05 μg/ml,0.1 μg/ml,0.5 μg/ml） and organic compounds concentrated by XAD-8 （10 ml/ml）,OTM were significantly increased compared with the corresponding alone exposure （P〈0.01）. When HepG2 ceils were co-exposed to MC-LR （0.1 μg/ml, 0.5 μg/ml） and organic compounds concentrated by XAD-2/8 （30 ml/ml）,OTM were significantly increased compared with the corresponding alone exposure （P〈0.01）. Factorial analysis revealed that co-exposure to organic compounds concentrated by the three resins and MC-LR had interaction effects （P〈0.01）. Conclusion There is a synergistic effect between organic extracts from tap water and MC-LR. MC-LR may reinforce the effect of DNA damage induced by organic extracts from drinking water.

关 键 词：水 大孔吸附树脂 有机提取物 微囊藻毒素 DNA损伤 彗星试验 联合作用 

分 类 号：R994.6[医药卫生—毒理学]