汉坦病毒G2糖蛋白多表位抗原基因的设计与构建  

Design and Construction of G2 Glycoprotein Multi-epitope Antigen Gene of Hantavirus

作　　者：杨东靖[1,2,3] 陈锦英[3] 苏旭[1,2] 李力[1,2] 吕莉琨[1,2] 刘勇[1,2] 

机构地区：[1]天津市疾病预防控制中心病原生物检测所,天津300011 [2]天津市预防医学研究所,天津300011 [3]天津医科大学基础医学院,天津300070

出　　处：《环境与健康杂志》2010年第1期38-40,共3页Journal of Environment and Health

基　　金：天津市科技支撑计划重大项目(07SYSYSF05100)

摘　　要：目的构建汉坦病毒SEO型代表株L99G2蛋白的多表位抗原基因(mea)。方法通过生物信息学软件对L99株G2蛋白氨基酸序列进行综合分析及预测,优选B细胞表位,引入GPG间隔序列串联表位,设计mea,然后应用重叠PCR法构建mea,并将其克隆到原核表达质粒pET32a(+)。结果优选出5个B细胞表位,设计并成功构建mea,PCR定向克隆获得pET32a-mea重组表达质粒。结论首次构建了汉坦病毒G2糖蛋白mea及其原核表达系统E.coliBL21/pET32a-mea,为其表达及免疫学应用奠定基础。Objective To construct the muhi-epitope antigen （mea） gene of G2 glycoprotein of Hantavirus SEO type L99 strain. Methods The B cell epitopes was chosen and connected by three-peptide GPG after the amino acid sequence of G2 protein was analyzed and predicted by bioinformatical soft wares. The corresponding gene mea was constructed by overlap PCR, and cloned into the prokaryotic expression plasmid pET32a （＋）. Results The mea gene was successfully constructed by the five epitopes being chosen. The recombinant plasmid pET32a-mea was acquired by directional cloning. Conclusion The mea and its expression system E.coli BL21/pET32a-mea were constructed for the first time. The foundation was laid for the expression of the mea and its immunological applications.

关 键 词：基因 汉坦病毒 B细胞表位 多表位抗原 

分 类 号：R373.3[医药卫生—病原生物学]