地卓西平马来酸盐和牛磺酸对甲基汞致大鼠脑谷氨酸代谢的影响  

Antagonism of Dizocilpine Maleate and Taurine to Methylmercury-induced Glutamate Metabolism Disturbance in Cerebrum of Rats

作　　者：邓小强[1] 徐兆发[1] 辛辛[1] 田雅文[1] 刘巍[1] 陈蓉[1] 

机构地区：[1]中国医科大学公共卫生学院环境卫生学教研室,辽宁沈阳110001

出　　处：《环境与健康杂志》2010年第1期41-44,共4页Journal of Environment and Health

摘　　要：目的研究地卓西平马来酸盐(dizocilpine maleate,MK-801)和牛磺酸对大鼠甲基汞中毒所致的谷氨酸代谢紊乱的影响。方法将健康清洁级Wistar大鼠40只按体重随机分为对照组、低剂量甲基汞染毒组、高剂量甲基汞染毒组、MK-801+甲基汞染毒组和牛磺酸+甲基汞染毒组。,每组8只。对照组和低、高剂量甲基汞染毒组皮下注射生理盐水,MK-801+甲基汞染毒组和牛磺酸+甲基汞染毒组分别皮下注射0.3μmol/kgMK-801和1mmol/kg牛磺酸。注射2h后,对照组腹腔注射0.9%生理盐水,低剂量甲基汞染毒组腹腔注射4μmol/kg氯化甲基汞,高剂量甲基汞染毒组、MK-801+甲基汞染毒组和牛磺酸+甲基汞染毒组腹腔注射12μmol/kg氯化甲基汞。连续进行4周,每周5天,每天染毒氯化甲基汞1次,每周一、三、五注射MK-801和牛磺酸。测定各组大鼠大脑皮质汞含量、谷氨酸(Glu)和谷氨酰胺(Gln)含量以及谷氨酰胺合成酶(GS)和谷氨酰胺酶(PAG)活力。结果与对照组比较,高、低剂量甲基汞染毒组和MK-801+甲基汞染毒组、牛磺酸+甲基汞染毒组大鼠大脑皮质中汞含量均升高;高剂量甲基汞染毒组大鼠大脑皮质中Glu含量较高,GS活力较低;高、低剂量甲基汞染毒组大鼠大脑皮质中Gln含量较低,PAG活力较高,差异有统计学意义(P<0.01)。与高剂量甲基汞染毒组比较,MK-801+甲基汞染毒组和牛磺酸+甲基汞染毒组大鼠大脑皮质中汞含量无明显差异,Glu含量和PAG活力较低,Gln含量和GS活力较高,差异有统计学意义(P<0.01)。结论MK-801和牛磺酸对甲基汞所致大鼠谷氨酸代谢紊乱有一定的拮抗作用。Objective To determine the antagonism of dizocilpine maleate （MK-801） and taurine to glutamate metabolism disturbance induced by methylmercury （MeHg） in cerebrum. Methods Forty Wistar rats were randomly divided into five groups. The first group was the control group,the second one was low dose MeHg-exposed group and the third was high dose MeHg-exposed group, these three groups were given physiological saline respectively.The fourth group was subcutaneously injected with 0.3 μmol/kg of MK- 801 and the five group was subcutaneously injected with 1 mmol/kg of taurine.Two hours later,the control group was intraperitoneally given physiological saline, the second group was intraperitoneally given 4 μmol/kg of methylmercury chloride,the third, fourth and five groups were given 12 μmol/kg of methylmercury chloride.The administration above was given five times a week for 4 weeks. The contents of mercury and Glu and Gin and the activities of GS and PAG in pallium were determined. Results Compared with the control group, the activities of PAG and the contents of mercury and Glu in MeHg-exposed groups increased significantly,the activities of GS and the contents of Gln decreased significantly. Compared with high dose MeHg group,in MK-801 and taurine treated groups,no significant differences were seen in the contents of mercury,and the contents of Glu, the activities of PAG were decreased significantly,the contents of Gin and the activities of GS were increased significantly. Conclusion Methylmercury can disorder glutamate metabolism in cerebrum of rats, and MK-801 and taurine can effectively counteract the adverse effects of methylmercury.

关 键 词：金属 重 甲基汞 地卓西平马来酸盐 牛磺酸 谷氨酸代谢 

分 类 号：R994.6[医药卫生—毒理学]