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作 者:孙佳佳[1] 张其安[2] 江力[1] 方凌[2] 孙泽华[1] 汪洋[1] 任永兵[1] 吕申超[1] 曹树青[1]
机构地区:[1]合肥工业大学生物与食品工程学院,安徽合肥230009 [2]安徽省农业科学院园艺研究所,安徽合肥230031
出 处:《安徽农业科学》2010年第5期2315-2317,共3页Journal of Anhui Agricultural Sciences
基 金:国家科技支撑计划项目(2008BADC0B00);国家大学生创新性实验计划项目(081035917);安徽省国际合作项目(0808-0703002)
摘 要:[目的]研究自然条件下选育的3种不同耐寒番茄品系的耐寒指标及其生理特性。[方法]以苗龄21~28 d的番茄幼苗为材料,通过实验室冷处理模拟外界环境条件,鉴定植株的存活及生长状况;研究植株离体叶片冷处理恢复后的形态变化并测定相对电导率,鉴定不同品系番茄植株的耐寒程度,分析电导率与存活率的相关性;测定冷胁迫条件下番茄植株体内可溶性糖和脯氨酸含量变化。[结果]不同品系的番茄冷处理后,叶片形态变化、电导率大小、恢复后的生长状况存在较大差异;不同品系番茄植株体内可溶性糖含量变化差异显著,脯氨酸含量变化差异不显著。[结论]冷处理条件下不同品系番茄叶片的电导率与存活率存在一定相关性,植株耐寒性与可溶性糖含量的积累有关。[Objective] The aim was to study cold-resistant indexes and physiological characters of three different cold-resistant tomato strains selected under natural conditions.[Method] With 21-28 days tomato seedlings as tested materials,through simulating external environment conditions by the cold treatment in lab,the survival ratio and growth status of seedlings were identified;by studying the morphological change of vitro leaves after cold treatment and determining the relative conductivity,the cold-resistant degree of different tomato strains was identified and the correlation between conductivity and survival rate was analyzed;the variation of soluble sugar and proline content in plant of tomato was determined under the condition of cold stress.[Result] The results showed that leaf morphological changes,size of conductivity and growth status of different tomato strains after cold treatment have significant differences;the variation of soluble sugar contents in plant has significant differences,while the variation of proline content has no significant differences.[Conclusion] There is a certain correlation between conductivity and survival rate of different tomato leaves under cold treatment conditions,and plant cold tolerance is related to accumulation of soluble sugar content.
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