植物黄烷酮3-羟化酶的生物信息学分析  

Bioinformatic Analysis of Flavanone 3-hydroxylase in Plants

作　　者：李鹏[1,2] 饶灿[1,2] 彭江[1,2] 李想韵[1,2] 宋锋[1,2] 孙一铭[1,2] 孙敏[1,2] 

机构地区：[1]西南大学生命科学学院,重庆400715 [2]三峡库区生态环境教育部重点实验室,重庆400715

出　　处：《安徽农业科学》2010年第6期2817-2819,2823,共4页Journal of Anhui Agricultural Sciences

基　　金：三峡库区生态环境教育部重点实验室开放基金项目(EF20-0609)

摘　　要：研究黄烷酮3-羟化酶家族的酶学特性,为黄酮类化合物生物合成的分子机理提供理论依据。对NCBI已注册的12个植物F3H的核酸和氨基酸序列进行生物信息学方法分析,对其组成成分、理化性质、亚细胞定位、跨膜结构、分子进化、蛋白质二级结构和结构域进行预测和推断。结果得出,F3H蛋白是定位于细胞基质,无信号肽的非分泌型蛋白,α-螺旋和不规则卷曲是蛋白质二级结构中量最多的结构元件,伸展片段散布于整个蛋白质中。The research aimed to study the enzymatic characteristics of flavanone 3-hydroxylase and provide theoretical basis for the the molecular mechanism on flavonoid biosynthesis.By bioinformatic methods and tools,about flavanone 3-hydroxylase genes as well as the composition of amino acid sequences,molecular structure,physical and chemical characters,subcellular localization,transmembrane helices,molecular phylogenetic evolution,secondary and tertiary structure of the F3Hs from NCBI were analyzed and predicted.The results demonstrated that F3H was a non-secretory protein without signal peptide.The main motifs of predicted secondary structures of the protein were alpha helix and random coil,and extended strand were spreaded in the whole secondary structure.

关 键 词：F3H 生物信息学 结构与功能 序列分析 

分 类 号：S312[农业科学—作物栽培与耕作技术]