奶牛乳腺上皮细胞的分离培养及分子生物学鉴定  

Isolation and Culture and Molecular Biological Identification on Cows Mammary Epithelial Cells

作　　者：刘玮[1,2] 董雅娟[1,2] 柏学进[1,2] 王文明[1,2] 张守宝[1,2] 董懿为[1,2] 

机构地区：[1]青岛农业大学动物胚胎工程中心,山东青岛266109 [2]山东省黑牛繁育工程技术研究中心,山东青岛266109

出　　处：《安徽农业科学》2010年第6期2877-2879,共3页Journal of Anhui Agricultural Sciences

基　　金：国家"863"重点项目(2007AA100505);山东省重大科技专项资助(2007ZHZX11101)

摘　　要：利用贴壁筛选法分离培养奶牛乳腺上皮细胞,并对其进行核型分析和RT-PCR鉴定。结果表明,体外培养的奶牛乳腺上皮细胞生长旺盛,具有典型的上皮细胞形态特征,体外传代15次后核型未发生改变。RT-PCR鉴定结果表明,分离培养的细胞明显表达奶牛乳腺上皮细胞的2种特异性基因,即as1酪蛋白基因(1 736 bp)和k酪蛋白基因(780 bp)。The Cows mammary epithelial cells were isolated and cultured by adherent screening method,their karyotype was analyzed and they were identified by RT-PCR.The results showed that the cultured cows mammary epithelial cells grown well with the typical morphological characteristics of epithelial cells,and its karyotype had no change after 15 times of subculture in vitro.The identification results of RT-PCR showed that the cultured cells obviously expressed 2 kinds of specific gene of cows mammary epithelial cells,those were as1 casein gene（1 736 bp） and k casein gene（780 bp）.

关 键 词：乳腺上皮细胞 分离培养 鉴定 

分 类 号：S823[农业科学—畜牧学]