魟鱼软骨多糖的免疫调节作用  

Immune Regulation of Cartilage Polysaccharides in Stingray

作　　者：王富喜[1] 郭斌[2] 

机构地区：[1]辽宁医学院,辽宁锦州121001 [2]辽宁医学院附属第一医院,辽宁锦州121001

出　　处：《安徽农业科学》2010年第6期2979-2980,共2页Journal of Anhui Agricultural Sciences

基　　金：2008年辽宁省教育厅高等学校科研项目(2008393)

摘　　要：[目的]观察魟鱼软骨多糖对小鼠免疫器官胸腺和脾脏重量的影响以及脾脏组织细胞IL-2、IL-6、IFN-γ细胞因子的表达水平。[方法]腹腔注射环磷酰胺(CY)造成小鼠免疫抑制模型,以香菇多糖为阳性对照,魟鱼软骨多糖(低、中、高剂量),灌胃给药14 d,试验结束后称量胸腺和脾脏重量计算胸腺指数和脾指数,检测脾脏中细胞因子的表达情况:运用抗原对应抗体进行免疫组化SP法检测脾中IL-2、IL-6、IFN-γ细胞因子的表达水平。[结果]魟鱼软骨多糖能显著提高小鼠胸腺和脾脏重量,能拮抗环磷酰胺导致的免疫抑制,升高脾脏中细胞因子的表达水平从而提高机体的免疫功能。[结论]魟鱼软骨多糖对小鼠的免疫功能具有一定的调节作用,是一种良好的免疫调节剂。[Objective] The research aimed to observe the effects of cartilage polysaccharides in stingray on the weight of immune organs（thymus and spleen） and the expression levels of cytokines IL-2,IL-6,IFN-γ in the spleen cells of mice.[Method] The cyclophosphamide（CY） was given by intraperitoneal injection to make the immunosuppressed model of mice.Taking lentinan as a positive control,low,middle and high dosage of cartilage polysaccharide were given by intragastric administration for 14 days.The weight of thymus and spleen were weighed to calculate the thymus index and spleen index.The expression of cytokines in the spleen was detected.The antigens corresponding to antibodies were used to detect the expression level of IL-2,IL-6,IFN-γ in the spleen by using immunohistochemistry SP method.[Result] The cartilage polysaccharides in stingray could significantly enhance the weight of thymus and spleen in mice,antagonize the immune suppression caused by cyclophosphamide and increase the expression level of cytokines in the spleen to enhance the body＇s immune function.[Conclusion] The cartilage polysaccharides in stingray has some regulatory role in the immune function of mice.And it is a kind of good immunomodulator.

关 键 词：魟鱼 软骨多糖 免疫组化 细胞因子 

分 类 号：R285[医药卫生—中药学]