川芎嗪对大鼠肝脏缺血再灌注损伤的保护作用  

Protective effect of ligustrazine against ischemia-reperfusion injury in rat liver

作　　者：王振猛[1] 张鹏[2] 唐乙[3] 宋少华[4] 刘芳[4] 俞卫锋[1] 

机构地区：[1]第二军医大学东方肝胆外科医院麻醉科,上海200438 [2]第二军医大学长海医院检验科,上海200433 [3]第二军医大学长海医院基础医学部免疫学教研室,上海200433 [4]第二军医大学长征医院器官移植科,上海200002

出　　处：《药学服务与研究》2010年第1期30-33,共4页Pharmaceutical Care and Research

基　　金：上海市卫生局青年课题资助项目(No.2009Y064)

摘　　要：目的:研究川芎嗪对大鼠肝脏缺血再灌注损伤的保护作用及其机制。方法:SD大鼠随机分为5组:空白对照组、假手术组、缺血再灌注组、生理盐水组和川芎嗪组。其中空白对照组大鼠直接处死;假手术组开腹后60 min关闭腹腔;缺血再灌注组阻断70%肝血流60 min,再灌注4 h;生理盐水组予腹腔内注射生理盐水8 ml/kg,30 min后阻断70%肝血流60 min,再灌注4 h;川芎嗪组予腹腔内注射川芎嗪80 mg/kg,30 min后阻断70%肝血流60 min,再灌注4 h。速率法测血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)和天冬氨酸氨基转移酶(aspartate aminotransferase,AST)活性,酶联免疫吸附法(ELISA)测血白介素-1(IL-1)、白介素-10(IL-10)和肿瘤坏死因子-α(TNF-α)水平。苏木素-伊红染色观察肝脏病理改变。结果:川芎嗪组血清ALT、AST、IL-1和TNF-α水平均比缺血再灌注组和生理盐水组明显降低(P<0.05),而IL-10则明显高于缺血再灌注组和生理盐水组(P<0.05)。病理结果显示,川芎嗪组大鼠肝细胞损伤较缺血再灌注组和生理盐水组为轻。结论:川芎嗪对大鼠肝脏缺血再灌注损伤具有保护作用,其机制可能与抑制炎性细胞因子生成及促进抗炎细胞因子表达有关。Objective：To investigate the protective effect of ligustrazine against ischemia-reperfusion（IR） injury in rat liver and its mechanism.Methods：Thirty-eight male SD rats were randomly divided into control group（n=6）,sham-operation group（n=6）,ischemia-reperfusion group（n=10）,normal saline group（n=6） and ligustrazine group（n=10）.The rats in control group were sacrificed without any treatment.The rat in sham-operation group underwent a midline laparotomy for 60 min.The rats in ischemia-reperfusion group received a 60-min occlusion of 70% hepatic blood inflow,followed by 4 h reperfusion.The rats in normal saline group or in ligustrazine group received normal saline（8 ml/kg） or ligustrazine（80 mg/kg） intraperitoneally 30 min prior to occlusion of 70% hepatic blood inflow.After 4 h reperfusion,rats were sacrified.Serum activities of alanine aminotransferase（ALT） and aspartate aminotransferase（AST） were determined by rate method and the liver histological changes were examined after hematoxylin-eosin（HE） staining.Serum concentrations of tumor necrosis factor-α（TNF-α）,interleukin-1（IL-1） and interleukin-10（IL-10） were detected by enzyme linked immunosorbent assay（ELISA）.Results：Serum levels of ALT and AST,serum concentrations of IL-1 and TNF-α were markedly decreased（P0.05）,while IL-10 concentration was obviously increased（P0.05） in ligustrazine group compared with the ischemia-reperfusion group and normal saline group.The injury of hepatocytes was ameliorated in ligustrazine group compared with the ischemia-reperfusion group and normal saline group.Conclusion：Ligustrazine can reduce the ischemia-reperfusion injury of rat liver by inhibiting the expression of proinflammation cytokines and promoting anti-inflammation cytokine IL-10 expression.

关 键 词：川芎嗪 缺血再灌注 肝脏 大鼠 

分 类 号：R973[医药卫生—药品]