优选超临界CO_2萃取灵芝孢子粉中总三萜化合物的最佳工艺  

Optimal technology for extraction of total triterpenoid compounds from Ganoderma lucidum spores by supercritical CO_2 extraction

作　　者：陈燕[1] 柳正良[2] 陈志东[1] 陆瑶华[1] 郭澄[1] 

机构地区：[1]上海交通大学附属第六人民医院药剂科,上海200233 [2]第二军医大学药学院药物分析学教研室,上海200433

出　　处：《药学服务与研究》2010年第1期34-36,共3页Pharmaceutical Care and Research

摘　　要：目的:建立超临界CO2萃取法提取和纯化灵芝孢子粉中总三萜化合物的最佳工艺条件。方法:采用超临界CO2萃取技术,以正交试验考察提取压力、温度和时间对提取效果的影响。结果:最佳工艺条件为萃取压力22 MPa,萃取温度50℃,萃取时间2 h。总三萜化合物的回收率为17.6%,在灵芝孢子粉中含量为160 mg/g。结论:优选出的工艺稳定可靠,简便易行。Objective：To establish the optimal technology for extraction and purification of total triterpenoids from Ganoderma lucidum spores by supercritical CO2 extraction.Methods：The influence of different factors such as extraction pressure,temperature and time on the extraction efficiency was studied.The technique of supercritical CO2 extraction was optimized by orthogonal tests.Results：The optimal extraction technique was as follows：extraction pressure was 22 MPa,extraction temperature was 50 ℃,and extraction time was 2 h.The extraction efficiency of total triterpenoids compounds was 17.6% and the content of total triterpenoids in Ganoderma lucidum spores was 160 mg/g.Conclusion：The optimal technology is stable,reliable,convenient and practical for extraction of total triterpenoids from Ganoderma lucidum.

关 键 词：灵芝孢子粉 超临界CO2萃取 总三萜化合物 提取工艺 正交试验 

分 类 号：R943[医药卫生—药剂学] R979.1[医药卫生—药学]