深层发酵培养的虎奶菇多糖对3种小球藻的凝集作用  

Agglutination of Three Kinds of Chlorella Cells by Polysaccharides from Pleurotus tuber-regium by Submerged Fermentation

作　　者：刘艳如[1] 林勇[2] 郑怡[1] 蔡文凯[1] 吴岳远[1] 蔡玉凤[1] 林艺平[1] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108 [2]福建省农业科学院食用菌研究所,福建福州350014

出　　处：《福建师范大学学报（自然科学版）》2010年第2期106-110,共5页Journal of Fujian Normal University：Natural Science Edition

基　　金：福建省科技厅重点资助项目(2008N0034);福建省自然科学基金资助项目(2007J0326);福建省教育厅资助项目(JA07046)

摘　　要：以深层发酵培养的虎奶菇(Pleurotus tuber-regium)菌丝体和发酵液为材料,提取胞内和胞外多糖,研究对藻类细胞的凝集作用.结果表明:胞内多糖使蛋白核小球藻(Chlorella pyrenoidosa)和捷克小球藻(Chlorella luteorividis)产生凝集的最小凝集质量浓度分别是23.3,186.4μg/mL,而胞外多糖使上述2种小球藻产生凝集的最小凝集质量浓度分别是3.3,211.3μg/mL,对海洋小球藻(Chlorella sp.)无凝集反应.Extracellular and intracellular crude polysaccharides were extracted from Pleurotus tuber-regiurn under the culture of submerged fermentation and tested for agglutination with algal cells. The results showed that intracellular and extracellular crude polysaccharides could agglutinate Chlorella pyrenoidosa and Chlorella luteorividis, but they had no agglutination with Chlorella sp.. The minimal concentration of intracellular crude polysaccharides, which could agglutinate Chlorella pyrenoidosa and Chlorella luteorividis cell suspension, were 23.3,186.4 μg/mL , respectively. The minimal concentration of extracellular crude polysaccharides, which could agglutinate Chlorella pyrenoidosa and Chlorella luteorividis cell suspension, were 3.3, 211.3μg/mL, respectively.

关 键 词：虎奶菇 多糖 藻细胞 凝集作用 

分 类 号：Q936[生物学—微生物学]