三氧化二砷联合Trolox对人早幼粒白血病细胞HL-60增殖和凋亡的影响  

Effects of arsenic trioxide in combination with Trolox on proliferation inhibition and apoptosis in human promyelocytic leukemia HL-60 cells

作　　者：刘国安[1] 张亚楠[1] 杨玉玲[1] 闵建平[1] 苏怡丹[1] 杨红[1] 丁兰[1] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2010年第2期82-87,共6页Journal of Northwest Normal University(Natural Science)

基　　金：甘肃省教育厅研究生导师项目(0901-05);西北师范大学三期"知识与科技创新"团队项目(nwnu-kjcxgc-03-49)

摘　　要：利用MTT法、台盼蓝排染法、Hoechst33258荧光染色法、瑞氏-姬姆萨染色法以及单细胞凝胶电泳法检测了三氧化二砷(As2O3)单独作用及联合Trolox后对人早幼粒白血病细胞HL-60的增殖抑制、凋亡诱导作用及其DNA损伤作用.结果表明,1μmol.L-1As2O3作用即可抑制HL-60细胞的增殖,联合100μmol.L-1Trolox对HL-60细胞增殖抑制作用较As2O3单独作用时明显增强.高浓度的As2O3(4μmol.L-1)作用24h可引起HL-60细胞的凋亡,与Trolox联合作用,在低浓度(1μmol.L-1)下即可引起细胞凋亡.联合100μmol.L-1Trolox可以显著增强As2O3引起的HL-60细胞的DNA损伤.这种损伤可能导致细胞周期阻滞,从而在生长曲线中体现出对HL-60细胞的增殖抑制作用.The effects of arsenic trioxide alone and in combination with Trolox on proliferation and apoptosis of human promyelocytic leukemia HL-60 cells are studied using MTT assay, typanblue exclusion method, Hoechst 33258 stain assay, Giemsa staining and single celt gel electrophoresis separately. The results demonstrate that the inhibitory effect of arsenic trioxide in combination with 100 μmol·L^-1 Trolox is more significantly than arsenic trioxide alone on HL-60 cells. Arsenic trioxide induces apoptosis at a concentration of 4μmol·L^-1 for 24 h, while apoptosis appeares at a lower concentration of 1 μmol·L^-1 for 24 h in combination with 100 μmol·L^-1 Trolox. The DNA damage induced by combination of arsenic trioxide and 100μmol·L^-1 Trolox is more significantly than arsenic trioxide alone on HL-60 cells. The DNA damage may lead to cell cycle arrest, as a result, the growth curve shows an inhibitory effect on the proliferation of HL 60 cells.

关 键 词：AS2O3 TROLOX 人早幼粒白血病细胞 生长抑制 凋亡 

分 类 号：Q253[生物学—细胞生物学]