切口痛大鼠脑干嘴端腹内侧髓质谷氨酸和γ-氨基丁酸释放的动态变化  

Dynamic changes in glutamate and γ-aminobutyric acid release in the rostral ventromedial medulla in a rat model of incisional pain

作　　者：王云[1] 冯春生[2] 巫志国[3] 穆小波[1] 吴安石[1] 岳云[1] 

机构地区：[1]首都医科大学附属北京朝阳医院麻醉科,100020 [2]吉林大学第一医院麻醉科 [3]广东省英德市人民医院麻醉科

出　　处：《中华麻醉学杂志》2010年第1期47-49,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30801073）

摘　　要：目的探讨切口痛大鼠脑干嘴端腹内侧髓质谷氨酸和弘氨基丁酸释放的动态变化。方法雄性SD大鼠，体重250—300g，麻醉下于右侧脑干嘴端腹内侧髓质内置入微透析导引管。恢复5d后，选择12只无神经行为学障碍的大鼠，随机分为2组（n=6）：A组仅吸入1．2％异氟醚5min；B组吸入1．2％异氟醚5min，同时制备右足底切口痛模型。分别于术前（基础状态）、术后3h、1d,2d、3d时收集微透析液20μl，采用高压液相法测定微透析液内谷氨酸和弘氨基丁酸浓度，各神经递质的释放水平以各时点测定值与基础值的百分数表示。结果与基础值比较，B组术后1d时谷氨酸的释放水平升高（P〈0．01），术后2、3d时谷氨酸的释放水平差异无统计学意义（P〉0．05），术后3h、1d、2d,3d时γ-氨基丁酸的释放水平升高（P〈0．01）；与A组比较，B组术后1d时谷氨酸的释放水平升高，术后3h、1d,2d、3d时γ-氨基丁酸的释放水平升高（P〈0．01）。结论大鼠术后脑干嘴端腹内侧髓质谷氨酸和γ-氨基丁酸的释放增加，可能改变了下行疼痛调控通路的功能。Objective To investigate the time-dependent changes in glutamate （Glu） and T-aminobutyric acid （GABA） release in the rostral ventromedial medulla in a rat model of incisional pain. Methods Healthy male SD rats weighing 250-300 g were used in this study. Twelve rats in which microdialysis carmulae were implanted in the right rostral ventromedial medulla without neurological deficits were randomly divided into 2 groups （ n = 6 each） ： group A control and group B incisional pain. In group B an 1 cm long incision was made in the plantar surface of fight hindpaw under 1.2 % isoflurane anesthesia which was maintained for 5 min. Samples of dialysate were collected before incision （T0 ,baseline） and at 3 h, 1 d, 2 d and 3 d after incision was made （T1-4） in both groups for determination of Glu and GABA concentrations （by HPLC ）. Results In group B Glu and GABA concentrations in the dialysate were significantly increased at 1 d （T2） and 3 h-3 d （Tl-4） respectively as compared with the baseline value at To and were significantly higher than those in group A （control group）. Conclusion Incisional pain increases the release of Glu and GABA in the rostral ventromedial medulla which might influence the function of descending pain modulation pathway.

关 键 词：氨基酸类 疼痛 延髓 

分 类 号：R402[医药卫生—临床医学]