妥拉苏林分子印迹膜传感器的制备及识别特性研究  

Preparation and Characterization of Tolazoline Sensor Based on Molecularly Imprinted Polymer

作　　者：张进[1,2] 徐岚[1] 吕瑞红[1] 王亚琼[1] 

机构地区：[1]西南大学化学化工学院发光与实时分析教育部重点实验室,重庆400715 [2]贵州师范学院化学与生命科学学院,贵阳550018

出　　处：《化学学报》2010年第2期157-161,共5页Acta Chimica Sinica

基　　金：重庆市自然科学基金(No.2008BB4263)资助项目

摘　　要：用电聚合的方法在金电极上制备了以妥拉苏林为模板分子的自组装邻氨基硫酚分子印迹膜传感器.通过差分脉冲伏安法研究传感器对妥拉苏林的响应特性,结果表明在优化的实验条件下,此传感器的峰电流响应值与妥拉苏林浓度在0.4～5μg·mL-1(r=0.9986)和5～120μg·mL-1(r=0.9953)范围内保持良好的线性关系,检测限为0.18μg·mL-1;相对标准偏差约为2.47%,响应时间约为3min.传感器具备较好的选择性和稳定性,初步用于人体尿液中妥拉苏林的分析,获得了较满意的结果.A novel electrochemical sensor for the detection of tolazoline based on molecularly imprinted polymer of electropolymerized o-aminothiophenol onto a gold electrode was constructed.Its response to tolazoline was evaluated by differential pulse voltammmograms（DPV）.Under the optimized conditions,the response current of the proposed sensor decreased linearly in two concentration ranges of tolazoline from 0.4 to 5 μg·mL^-1（r=0.9986） and from 5 to 120 μg·mL^-1（r=0.9953） with a detection limit of 0.18 μg·mL^-1.The RSD（relative standard deviation） was about 2.47% and the response time was closed to 3 min.Moreover,the studied imprinted sensor exhibited high selectivity and long-term stability,and was successfully applied to the determination of tolazoline in human urine samples.

关 键 词：电化学传感器 分子印迹技术 电聚合物膜 妥拉苏林 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]