巨噬细胞移动抑制因子在不明原因早期复发性流产者血清、绒毛和蜕膜中的表达及意义  

Expression of Macrophage Migration Inhibitory Factor in Serum,Villi and Deciduas in Women with Unexplained Early Recurrent Spontaneous Abortion

作　　者：张钰[1] 李俊霞[1] 顾艳[1] 李奕[1] 

机构地区：[1]天津医科大学第二医院计划生育科,300211

出　　处：《中国计划生育学杂志》2010年第3期147-149,共3页Chinese Journal of Family Planning

摘　　要：目的:研究巨噬细胞移动抑制因子(MIF)在正常早孕和不明原因早期复发性流产血清、绒毛和蜕膜中的表达水平及在不明原因早期复发性流产(UERSA)发病中的作用。方法:选取2008年1～12月在天津医科大学第二医院计划生育科因不明原因早期复发性流产就诊的患者30例(UERSA组)以及同孕龄正常早孕妇女30例(正常早孕组),采用ELISA方法检测两组外周血清中MIF的浓度,并采用实时荧光RT-PCR检测两组绒毛、蜕膜组织中MIF-mRNA的表达水平,进行统计学比较。结果:UERSA组血清MIF水平(10.98±3.02ng/ml)低于正常早孕组(17.53±5.34ng/ml),绒毛、蜕膜组织中MIF-mRNA的2-△△CT值(0.022±0.001;0.97±0.58)均较正常早孕组(1.290±1.110;1.20±0.85)显著降低。经过相关分析,血清MIF水平与绒毛、蜕膜组织中MIF-mRNA的水平呈正相关(r=0.69,0.71)。结论:妊娠期母胎界面MIF表达降低,可能在不明原因早期复发性流产的发生发展中起重要作用。Objective： To explore the expression of macrophage migration inhibitory factor（MIF） in serum,villi and deciduas in women with normal early pregnancy and unexplained early recurrent spontaneous abortion,and the role of MIF contributing to the occurrence of unexplained early recurrent spontaneous abortion.Methods： Thirty women with unexplained early recurrent spontaneous abortion（the UERSA group） and 30 women with normal early pregnancy（the normal pregnant group） were recruited in the Second Hospital of Tianjin Medical University between January 2008 to December 2008.The serum MIF levels were assayed using enzyme-linked immunosorbent assay（ELISA） kits,and the expression of MIF mRNA in the villi and deciduas were determined by real-time fluorescence quantitative polymerase chain reaction.Results： The serum MIF level in the UERSA group（10.98±3.02ng/ml） was significant lower than that of the normal pregnant group（17.53±5.34ng/ml）.The expression of MIF mRNA in the villi and deciduas in the UERSA group（0.022±0.001;0.97±0.58） were significant lower than those of the normal pregnant group（1.290±1.110;1.20±0.85）.The serum MIF level had a positive correlation with the expressions of MIF mRNA in the villi and deciduas（r = 0.69,0.71）.Conclusion： The lower expression of MIF in villi and deciduas may contribute to the occurrence of unexplained early recurrent spontaneous abortion.

关 键 词：巨噬细胞移动抑制因子 复发性流产 绒毛 蜕膜 血清 

分 类 号：R714.21[医药卫生—妇产科学]