NF-κB及iNOS在猪腹部火器伤肠管穿透后心脏损伤中的作用  

Intestinal Perforations Due to Abdominal Firearm Wound in Pigs

作　　者：薛会朝[1] 郝利霞[1] 李泽信[1] 刘江伟[2] 冯德元[2] 李崎[2] 高伟[2] 

机构地区：[1]新乡医学院第一附属医院普外科,河南卫辉453100 [2]兰州军区乌鲁木齐总医院肝胆外科,新疆乌鲁木齐830000

出　　处：《河南职工医学院学报》2010年第1期1-3,F0003,共4页Journal of Henan Medical College For Staff and Workers

基　　金：兰州军区医药卫生科研基金资助项目(LXH-2005019)

摘　　要：目的探讨NF-κB、iNOS在腹部火器伤肠管穿透后心脏损伤中的作用。方法健康长白仔猪42头,随机分为对照组和伤后1 h,2 h,4 h,8 h,12 h和24 h组,用免疫组化图像分析法测定各组心肌内NF-κB、iNOS表达,在光镜和电镜下观察各组心肌组织形态学变化。结果伤后各组心肌内NF-κB、iNOS表达明显高于对照组。伤后8 h、12 h、24 h组光镜下出现逐渐加重的心肌细胞水肿、变性;电镜下4 h、8 h、12 h、24 h组出现逐渐加重的线粒体肿胀、溶解;对照组光镜及电镜下未见明显的损伤性变化。相关分析表明,伤后心肌组织NF-κB与iNOS呈正相关(r=0.980)。结论腹部肠管火器伤后可诱导NF-κB、iNOS表达并介导心脏损伤。Objective To investigate the role of NF -κB and iNOS in the mechanism of heart injury following intestinal perforations due to abdominal firearm wound. Methods 42 Chang-Bai piglets were randomized into 7 groups： control group and 1 hour group, 2 hour group, 4 hour group, 8 hour group, 12 hour group and 24 hour group after being wounded. The model of intestinal perforations due to abdominal firearm wound was established in wounded groups. The expression of cardiac NF -κB and iNOS were determined by immunohistochemical staining and image analysis. The histological change of cardiac tissue was observed with light and electron microscope. Results The expression of cardic NF -κB, iNOS in wounded groups significantly increased in comparison with the control group. The experimental group of 8 h, 12 h, 24 h appeared gradually aggravated cardiac cells edema and degeneration under light microscope, mitochondrial appeared gradually aggravated swelling and dissolved under EMS in 4 h, 8 h, 12 h, 24 h groups. There was no obviously change of injury in the control group under fight microscope and EMS. Correlation analysis showed that the expression of iNOS in myocardial tissue was positively correlated with NF -κB （r = 0. 980）. Conclusion The NF -κB, iNOS in myocardial cell can be induced by intestinal firearm wound and mediates the heart injury.

关 键 词：NF-ΚB INOS 腹部火器伤 心脏损伤 

分 类 号：R541[医药卫生—心血管疾病]