十四烷基二甲基苄基氯化铵存在下1,2-萘醌-4-磺酸和氨茶碱显色反应及氨茶碱测定  

Sensitization of Tetradecy Benzyl Dimethyl Ammonium Chloride for the Color Development Reaction Between Aminophylline and Sodium 1,2-Naphthoquinone-4-Sulfonate and the Determination of Aminophylline in Pharmaceutical samples

作　　者：牛建平[1] 李晶[1] 张欢欢[1] 李全民[1] 

机构地区：[1]河南师范大学化学与环境科学学院,河南省环境污染控制重点实验室,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2010年第1期121-123,共3页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省教育厅自然科学研究计划项目(2008B150011)

摘　　要：氨茶碱与1,2-萘醌-4-磺酸钠和十四烷基二甲基苄基氯化铵在pH=13.0的缓冲溶液中反应生成组成比为1∶2∶2红褐色产物(λmax=455nm),表观摩尔吸光系数ε=4.97×103L·mol-1.cm-1.氨茶碱的浓度在0.73～55μg·mL-1范围内与吸光度(A)呈现良好线性关系,线性回归方程为A=0.25841+0.0497C(×10-5mol.L-1),相关系数r=0.9956.回收率在96.5%以上.该方法用于测定注射液中氨茶碱的含量,结果满意.A rapid,simple and sensitive method for the determination of aminophylline （Amin） using sodium 1,2-naphthoquine-4-sulfonate （NQS） and tetradecyl benzyl dimethyl ammonium chloride （Zeph） is established in this paper. The method is based on the russety product formed from Amin,NQS and Zeph in a buffer solution of pH 13.00,and the stoichiometric ratio of the product is 1∶2∶2. Beer＇s law is obeyed in a range of 0.73-55 μg·mL-1 of Amin at the maximum absorption of 455 nm （ε=4.97×103 L·mol-1cm-1）.The linear regression equation of the calibration graph is A=0.258 41＋0.049 7C,with a linear regression correlation coefficient of 0.995 6. The detection limit is 0.7 μg·mL-1,R.S.D. 1.1% and average recovery rateis in range of 96.5%-103%.This method has been successfully applied to the determination of Amin in pharmaceutical samples.

关 键 词：十四烷基二甲基苄基氯化铵 1  2-萘醌-4-磺酸钠 氨茶碱 

分 类 号：O657.32[理学—分析化学]