羊耳菊有机溶剂提取物的抑菌作用研究  

Antimicrobial Effects of Inula Cappa Organic Solvent Extracts

作　　者：刘胜贵[1,2] 陈鸠鸠[1] 李军[1] 陈富成[1] 邓乐[2] 

机构地区：[1]怀化学院生物工程系,湖南怀化418008 [2]湖南师范大学生命科学学院,湖南长沙410081

出　　处：《辽宁中医杂志》2010年第3期398-400,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：湖南省教育厅科研项目(06C637)

摘　　要：目的:评价羊耳菊提取物抗微生物活性,为广泛应用于传统医药系统的药物提供科学依据。方法:通过使用不断增强极性的有机溶剂,即石油醚、氯仿、乙醇、甲醇、丙酮和冰醋酸,获得羊耳菊根、茎、叶的提取物。使用药敏纸片扩散法和试管二倍稀释法,研究了提取物对大肠杆菌(E.coliO1)、白色念珠菌(Candida albicans)、金黄色葡萄球菌(Staphy-lococcus aureus 91053)、甲型副伤寒沙门氏菌(Salmonella paratyphiA)、铜绿假单胞菌(Pseudomonas aeruginosa)、粪肠球菌(Enterococcus faecalis)的体外抑菌特性。结果:羊耳菊不同有机溶剂提取物都具有抑菌作用,其中,冰醋酸提取物的抑菌效果最好,其次是乙醇和丙酮提取物,石油醚提取物抑菌效果略差;根和叶提取物抑菌作用比茎提取物好;提取物对革兰氏阳性抑制效果比革兰氏阴性菌好。结论:羊耳菊有机溶液提取物体外试验证实了该民间药物具有抗菌活性,也表明了羊耳菊是一种有开发潜力的新抗菌药物资源。Objective：To evaluate the antimicrobial activity of extracts of this plant in order to provide scientific proof for it＇s wide application in traditional medicine system.Methods：Extraction of whole roots,stems and leaves of Inula cappa using solvents of increasing polarity,namely,petroleum ether,chloroform,ethanol,methanol,acetone and glacial acetic acid,respectively,were obtained.The extracts were tested for their in vitro antimicrobial properties by using a disk-diffusion and serial two-fold dilutions method.Results：All of the plant extracts tested had antimicrobial activities.The glacial acetic acid extracts exhibited a stronger and broader spectrum of antibacterial activity,followed by the ethanol and acetone extracts.Both root and leave extracts have stronger antimicrobial activities than stem extracts,and the plant extracts were more active against Gram-positive bacteria than against Gram-negative bacteria.Conclusion：This in vitro study corroborated the antimicrobial activity of the selected plants used in folkloric medicine and also showed that Inula Cappa DC.could be potential sources of new antimicrobial agents.

关 键 词：羊耳菊 提取物 抑菌圈直径 MIC值 

分 类 号：R284.2[医药卫生—中药学]