小鼠卵泡颗粒细胞分化不影响卵母细胞印迹基因DNA甲基化进程  

DNA Methylation of Imprint Genes in the Oocytes Was Not Affected by the Differentiation of Granulose Cells

作　　者：宋振华[1] 闵令江[1] 荣美洁[1] 潘庆杰[1] 沈伟[1] 

机构地区：[1]青岛农业大学动物科技学院动物生殖发育与基因工程研究所,山东青岛266109

出　　处：《青岛农业大学学报（自然科学版）》2010年第1期6-10,16,共6页Journal of Qingdao Agricultural University(Natural Science)

基　　金：国家863计划重点项目(2008AA101003);山东省自然科学基金(Z2007D01);"泰山学者"建设工程专项经费;青岛农业大学高层次人才启动基金重点项目(630615)

摘　　要：雌性生殖细胞印迹的获得发生于小鼠的卵子发生过程中,本研究以颗粒细胞分化前后的卵泡卵母细胞为研究对象,利用重亚硫酸盐测序法,对卵泡腔形成前后的卵母细胞中Igf2r和Peg3两个印迹基因的甲基化状况进行了分析。颗粒细胞分化前,卵母细胞Igf2r和Peg3两个印迹基因DMR区CpG位点的甲基化比率分别为80.67%和82.78%;颗粒细胞分化后的早期有腔卵泡卵母细胞中,卵母细胞Igf2r和Peg3两个印迹基因DMR区CpG位点的甲基化比率分别为82%和83.89%。可见,卵泡腔的形成对于直径相同的卵泡卵母细胞印迹基因甲基化的建立没有影响。The acquisition of methylafion imprints occurred in oogenesis process in mouse female germ ceils. In this study, the oocytes which derived from preantral and antral follicles in 16 dpp mouse were studied using bisulfite sequencing method, the methylation states of imprinting genes（ Igf2r and Peg3 ） were analyzed. The results showed that the percentage of methylated CpG sites on the Igf2r and Peg3 DMRs were 80.67% and 82.78% in the prean- tral follicles, respectively. Compared to the preantral follicles, there were 82% and 83.89% in the antral follicles. These data indicated that the formation of follicular cavity does not regulate the establishment of methylation imprint in oocytes.

关 键 词：小鼠 卵泡 卵母细胞 印迹基因 甲基化 

分 类 号：S814.4[农业科学—畜牧学]