磁性粒子在蛋白质分离纯化中的应用  

Applications of Magnetic Particles in Proteins Separation and Purification

作　　者：盖青青[1] 屈锋[1] 梅芳[1] 张玉奎[2,3] 

机构地区：[1]北京理工大学生命科学学院,北京100081 [2]中国科学院大连化学物理研究所,大连116023 [3]中国科学院

出　　处：《化学通报》2010年第2期99-105,共7页Chemistry

基　　金：国家重点基础研究发展计划项目(2007CB914101);国家自然科学基金项目(20875009)资助

摘　　要：磁性粒子与磁性分离技术结合用于生物分子和生物微粒的分离近年来受到了广泛关注。磁性粒子经过适当的表面修饰,可高度选择性地结合目标蛋白,在解决蛋白质的快速分离及高特异性的选择性分离方面具有优势。随着蛋白质组学研究的发展,磁性粒子用于蛋白质分离纯化的研究日渐增多。本文介绍了磁性粒子和磁性分离的基本特点,综述了近5年国内外有关磁性粒子在蛋白质分离纯化中的应用和发展现状。The application of magnetic particles in combination with magnetic separation for biological molecules and bio surface logical particles has attracted considerable attention in recent years. The magnetic particles with suitable modification are capable of binding proteins selectively. Magnetic particles and the magnetic separations have advantages of rapidity, convcnie particles are increasingly nce and high selectivity proteins separation and With the fast development of proteomics research, magnetic magnetic particles and magnetic separation are introduced and the particles applied in proteins separation and purification are reviewed. In this paper, the fundamental characteristics of recent five years＇ development of magnetic

关 键 词：磁性粒子 蛋白质 分离纯化 

分 类 号：Q51[生物学—生物化学]