灯盏花素在丙醇-硫酸铵双水相体系中的分配行为研究  

Study on the Partition Characteristics of Breviscapinum in the Propyl-Alcohol Ammonium Sulfate Aqueous Two-phase System

作　　者：高云涛[1] 戴云[1] 李世月[1] 杨耀洪[1] 

机构地区：[1]云南民族大学化学与生物技术学院,云南昆明650031

出　　处：《云南民族大学学报（自然科学版）》2010年第2期106-109,115,共5页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：云南省社会发展科技计划项目(2007B148M);云南省教育厅科学研究基金(06Y195C);国家民委-教育部共建民族药资源化学重点实验室开放基金(MJY07010)

摘　　要：研究了灯盏花素在丙醇-硫酸铵双水相体系中的分配行为,重点对双水相的形成、硫酸铵用量和初始pH值对分配比的影响进行了研究.在盐水相、丙醇初始体积分别为10.0 mL和4.0mL,硫酸铵用量为3.5 g,初始pH为4.08条件下,灯盏花素的最大分配比达107.5,相应的萃取率达97.7%.在实验基础上建立了相应的"二元弱酸"分配模型.Partition characteristics of breviscapinum in the propyl - alcohol ammonium sulfate aqueous two - phase system were studied. The formation of aqueous two -phase was investigated and the effects of the amount of （NH4）2SO4 and initial pH of the solution on the partition ratio were measured. The experiment showed that opti- mum partition initial conditions were 10.0 mL, salt - water volume and 4.0 mL propyl - alcohol volume, 3.5g （NH4） 2S04 and pH 4.08, with the maximum partition ratio as 107.5 and the maximum extraction rate as 97.7%. A ＂weak diaeid partition model＂ was developed based on the experiment.

关 键 词：灯盏花素 丙醇-硫酸铵双水相 分配比 

分 类 号：O657.7[理学—分析化学]