Ce(NO_3)_3对盾叶薯蓣茎段愈伤组织生长和不定根诱导影响的研究  

Effect of Ce(NO_3)_3 on Callus Growth and Adventitious Root of Stem From Dioscorea Zingberensis

作　　者：王建安[1] 沙莎[2] 李艳芝[1] 徐增莱[3] 吴国荣[2] 

机构地区：[1]济宁医学院药学院,山东日照276826 [2]南京师范大学生命科学学院,江苏南京210046 [3]江苏省中国科学院植物研究所,江苏南京210014

出　　处：《南京师大学报（自然科学版）》2010年第1期94-97,共4页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：科技部项目(2003DEB6J074);江苏省科技厅项目(BG2001045)

摘　　要：为研究硝酸铈对盾叶薯蓣快速繁殖的影响，采用以盾叶薯蓣茎段为外植体，在其愈伤诱导培养基、丛生芽培养基及生根培养基中添加不同浓度的Ce3＋结果表明，含Ce3＋为5mg／L的培养基可显著提高愈伤组织诱导率，含Ce3＋1mg／L的培养基丛生芽诱导率最高；1—15mg／L的Ce3＋对盾叶薯蓣组培苗生根有明显的促进作用，5mg／L的Ce3＋显示出最强的促进效应．可以认为，一定浓度Ce3＋对诱导愈伤组织生长、丛生芽萌发及不定根生长有促进作用，但高浓度的Ce3＋均对其呈现抑制效应．To study the effect of rare earth Ce（ NO3 ）3 on rapid clonal propagation of Dioscorea zingiberensis. Using stems of Dioscorea zingiberensis as explants, different concentrations of Ce （NO3 ）3 were supplemented to the medium （1/2 MS ＋ 0. 5 μmol/L IBA） to study the effect of Ce3 ＋ on the callus induction medium, adventitious bud initial medium and adventitious root medium. The callus induction was stimulated by 5.0 mg/L Ce3 ＋ ; 1.0 m g/L Ce3 ~ stimulated the bud initial induction; while 1 -15 mg/L stimulated adventitious root. The callus induction adventitious bud initial and roo- ting was stimulated by the low concentrations of rare earth elements, but higher concentration inhibited them.

关 键 词：铈 盾叶薯蓣 愈伤组织 不定根 

分 类 号：S282.1[农业科学—农业水土工程]