荧光法研究山莓叶提取物清除羟自由基的活性  

Study on Scavenging Activity of Extracts from Rubus Corchorifolius L. f. Leaves to Hydroxyl Free Radicals by Fluorimetry

作　　者：张敏[1,2] 胡文彬[1] 曹庸[3] 陈雪香[3] 

机构地区：[1]中南林业科技大学材料科学与工程学院,长沙市韶山南路498号410000 [2]湖南农业大学生物科学技术学院,长沙市410000 [3]华南农业大学食品学院,广州市510642

出　　处：《光谱实验室》2010年第2期413-417,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：湖南省教育厅重点科技计划项目(06A057)

摘　　要：在稀硫酸介质中,荧光染料亮绿(LGSF)在312nm处产生荧光,Fenton反应产生的.OH与亮绿反应,使其荧光猝灭。根据此原理,荧光光度法测定山莓叶不同萃取物清除自由基活性存在很大差异。同时,当提取物浓度达到0.40mg/mL时自由基清除率分别可达到27.34%,38.65%,40.22%,41.68%,其中,山莓叶正丁醇萃取物清除羟自由基活性最强。本研究为山莓叶的合理开发利用提供理论依据。The fluorescence can be quenched by the hydroxyl radical produced by fenton reaction. The free radical scavenging activity of Rubus corchorifolius L. f. was determinated by mearsuring the change of fluorescence intensity of LGSF. The extracts scavenging rates are 27. 34%, 38.65%, 40.22 %, 41.68% respectively when extract＇s concentration is 0.40mg/mL,and free radical scavenging capacities of EtOAC extract is the best. A reference method was provided for reasonable exploitation and utilization of Rubus corchorifolius L. f. leaves.

关 键 词：山莓叶 羟自由基 清除 荧光光度法 亮绿 

分 类 号：O657.32[理学—分析化学]