酶循环荧光法定量检测人血红细胞NADH浓度  

Determinaton of Human Red Blood Cells' NADH by Enzyme Cycling Fluorimetry

作　　者：刘洲君[1] 李启富[1] 蒋袁娟[1] 黄佳祎[1] 成丹[1] 

机构地区：[1]重庆医科大学附属第一医院内分泌科,重庆市渝中区友谊路1号400016

出　　处：《光谱实验室》2010年第2期602-606,共5页Chinese Journal of Spectroscopy Laboratory

摘　　要：用酶循环荧光测定法,多功能微孔板检测系统检测人血清还原型烟酰胺腺嘌呤二核苷酸(NADH)的浓度。通过底物和酶的循环反应将待测物质NADH的量扩大至荧光可检测范围。该方法的荧光强度与NADH的质量在0.00—0.80pmol范围内呈良好的线性关系,建立的线性方程式为:y=99.377x+13.481,r=0.9941,加标回收率为95.5%—100.0%,批内RSD为1.0%—2.7%,批间RSD为1.9%—12.6%。该方法准确度、灵敏度高、稳定性好,可用于NADH的定量检测。Enzyme cycling fluorimetry was applied to determine the concentration of reduced form of nicotinamide-adenine dinucleotid （NADH） in human red blood cells using multifunction MPP detection system. After circular reaction of the substrates and enzymes,the concentration of NADH arrived at the tested region. The calibration graph for the determination of NADH is in the range of 0. 00--0. 80pmol , with linear equation of y=99. 377x＋13. 481 ,r=0. 9941. The average recovery is 95.5%-100. 0%,intra assay RSD is 1.0%-2. 7% and inter assay RSD is 1.9%--12. 6%. The method is accurate ,sensitive and steady.

关 键 词：酶循环 荧光法 辅酶烟酰胺腺嘌呤二核苷酸 多功能微孔板检测系统 

分 类 号：O657.32[理学—分析化学]