Triton X-100敏化核固红与蛋白质作用的共振光散射光谱  

Study of Triton X-100 Sensitizing Effect on the Interaction of Nuclear Fast Red and Proteins by Resonance Light Scattering Spectrometry

作　　者：何全[1] 龙云飞[1] 李颖[1] 

机构地区：[1]湖南科技大学化学化工学院,湖南省湘潭市桃园路411201

出　　处：《光谱实验室》2010年第2期758-760,共3页Chinese Journal of Spectroscopy Laboratory

基　　金：湖南省自然科学基金资助项目(09JJ6021)

摘　　要：在pH=1.10的Tris-HCl缓冲溶液中,Triton X-100能增加有机染料核固红和蛋白质之间的相互作用,使共振光散射(RLS)强度增强。考察了影响因素,在优化条件下RLS强度增加值与蛋白质浓度之间呈良好的线性关系。该法具有灵敏度高、操作简单、准确度好、线性范围宽等特点,用于合成样品的测定,结果满意。The characteristics of interaction between proteins and nuclear fast red were studied by resonance light scattering （RLS） method in the pH of 1. 1 medium, at the presence of Triton X-100. A new method for determination of the proteins was established. This method has good operation, high sensitivity ,good accuracy and wide linear range. Synthetic samples were analyzed with satisfactory results.

关 键 词：核固红 蛋白质 TRITON X-100 共振光散射光谱 

分 类 号：O657.32[理学—分析化学]