高效液相色谱法测定硫酸特布他林与红细胞膜表面β2肾上腺素受体结合量  

Determination of Terbutaline Sulfate and Its Combination with β2 Adrenergic Receptor in Erythrocyte Membrane by High Performance Liquid Chromatography

作　　者：蒋心惠[1] 周岐新[1] 

机构地区：[1]重庆医科大学药学院,重庆400016

出　　处：《分析化学》2010年第3期377-380,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.30572353)资助项目

摘　　要：建立了高效液相色谱同时检测红细胞混悬液中硫酸特布他林与盐酸普萘洛尔的方法。红细胞与特布他林在37℃水浴振荡保温反应1h后,没有与细胞膜受体结合的特布他林通过1500r/min离心5min后分离,取上清液与受体-药物复合物分离。色谱柱为C18柱,以0.020mol/LKH2PO4溶液(含0.25%三乙胺,pH5.1)-甲醇(20∶80,V/V)为流动相,在荧光激发波长280nm,发射波长320nm处测定样品含量。在优化的条件下,特布他林与普萘洛尔在0.010～3.000mg/L范围内线性关系良好,相关系数分别为0.9999和0.9998。测定重复性相对标准偏差为0.8%和1.2%;检出限分别为1.0和3.0μg/L。该方法安全、简便、灵敏,适用于测定红细胞中硫酸特布他林和受体配体结合实验的研究。A high performance liquid chromatographic method with hypersil C18 column,0. 020 mol/L potassium dihydrogen phosphate solution（ which contains 0. 25% triethylamine,pH 5. 1）-methanol（ 20∶80,V/V） as mobile phase and fluorescence detection with 280 nm as excitation wavelength and 320 nm as emission wavelength has been developed for the determination of terbutaline sulfate and propranolol hydrochloride in Erythrocyte suspensions. Erythrocyte surface receptor oscillating reacted 1 h with terbutaline in 37 ℃ water bath. Free terbutaline and propranolol were separated from the membrane receptor-binding complex through 1500 r/min centrifugal in 5 min. The linear range was 0. 010-3. 000 mg/L in Alsever＇s solutions and the correlation coefficients were 0. 9999 and 0. 9998. The relative standard deviations（ RSDs） of terbutaline and propranolol were 0. 8% and 1. 2% ,respectively. The limits of detection（ LODs） of both were 1. 00 and 3. 00 mg/L. This simple,safety and sensitive method was suitable for the determination of terbutaline and propranolol in erythrocyte suspensions and for the study of receptor-ligand binding.

关 键 词：硫酸特布他林 盐酸普萘洛尔 红细胞膜 Β2肾上腺素受体 高效液相色谱 

分 类 号：R96[医药卫生—药理学]