人类博卡病毒结构蛋白基因VP2的克隆与表达  

Cloning and Expression of Structural Gene VP2 of Human Bocavirus

作　　者：陈莹[1] 张丹[1] 李京京[1] 李毅[1] 

机构地区：[1]华中师范大学生命科学学院昆虫学研究所,湖北武汉430079

出　　处：《化学与生物工程》2010年第3期67-69,共3页Chemistry & Bioengineering

摘　　要：采用PCR方法扩增出人类博卡病毒结构蛋白基因VP2,通过双酶切、连接、转化等方法将VP2基因克隆到原核表达载体pMAL-c2x上,构建重组质粒pMAL-c2x-VP2,通过双酶切检测重组质粒构建成功;用0.8 mmol.L-1IPTG诱导融合蛋白表达,经SDS-PAGE检测、Western Blot分析,证明目的蛋白得到了表达。可为目的蛋白的纯化及结构和功能的研究、相应抗体的制备打下坚实的基础。In this report, human bocavirus structural gene VP2 was amplied by using PCR approach, and the PCR product was cloned into the prokaryotic expression vector pMAL-c2x to generate the recombinant plasmid pMAL-c2x-VP2 and expressed in Escherichia coli DHSa strain. The maximal expression level of the fusion protein was reached with the induction of 0.8 mmol · L^-1 IPTG at 37℃ for 4 hours. The fusion protein was identified as target protein by Western Blot analysis with MBP-tag antibody. The purified protein provides an useful tool for the studies of its function.

关 键 词：人类博卡病毒 结构蛋白基因VP2 克隆 表达 

分 类 号：R373.1[医药卫生—病原生物学]