β-1,3-葡聚糖对感染沙门菌鸡免疫功能和肠道菌群数量的影响  

Effect of Dietary β-1,3-glucan on Immune Functions and Intestinal Microflora Levels of Broilers Challenged with Salmonella pullorum

作　　者：王忠[1] 强文军 呙于明[1] 王岩屾[1] 王莹[1] 

机构地区：[1]中国农业大学动物科技学院动物营养国家重点实验室,北京100193 [2]甘肃省武威市凉州区畜牧兽医服务中心,甘肃武威733000

出　　处：《中国家禽》2010年第5期14-18,共5页China Poultry

基　　金：国家"十一五"科技支撑计划(2007BAQ01047)

摘　　要：为探讨β-1,3-葡聚糖对感染鸡白痢沙门菌鸡免疫机能和肠道微生物菌群的影响。选择120只1日龄AA肉公鸡,随机分成4个处理组,每个处理组30只鸡。处理组C1和C2饲喂基础日粮;处理组T1和T2饲喂基础日粮+0.01%β-1,3-葡聚糖。在10日龄,处理组C2和T2鸡口服感染0.5 mL鸡白痢沙门菌(浓度为1×109CFU/mL),试验到21日龄结束。结果表明,与对照组相比,日粮添加β-1,3-葡聚糖有促进肉鸡生长,阻止沙门菌感染引起的肉鸡死亡率升高的趋势;日粮添加β-1,3-葡聚糖对沙门菌感染鸡(T2组)可显著下降外周血T淋巴细胞增殖活性(P<0.05)和小肠sIgA分泌量(P<0.05),显著降低回肠和盲肠沙门菌和大肠杆菌数量(P<0.05)。One hundred and twenty 1-day-age male commercial AA broilers were randomly distributed into four treatments with thirty broilers per treatment to evaluate the efficacy of dietary β-1,3-glucan against Salmonella pullorum （SP） in broilers. Each treatment was respectively reared in an environmentally controlled isolator. Treatments were：C1 （negative control,no SP challenge,basal diet）,C2（positive control,SP challenge, basal diet）,T1 （no SP challenge＋basal diet supplemented with 100 mg/kg β-1,3-glucan）and T2 （SP challenge＋basal diet supplemented with 100mg/kg β-1,3-glucan）. The broilers in treatment 2 and 4 were challenged SP at 10-day-age. The results showed ,that dietary β-1,3-glucan could hold back the trend of body reducing and mortality increasement due to the challengement of SP. Significantly lower peripheral blood T lympocytes proliferation index and intestinal sIgA levels were detected in T2（P〈0.05）. In addition,T2 had lower Salmonella and E.coli levels in ileum and caecum compared to C2（P〈0.05）.

关 键 词：鸡白痢沙门菌 口服感染 葡聚糖 肠道菌群 免疫功能 外周血T淋巴细胞 肠道微生物菌群 10日龄 

分 类 号：S858.315.1[农业科学—临床兽医学]