Ri质粒介导双价抗真菌病基因转化番茄的初步研究  

Study on Genetic Transformation of Tomato Expressing Bivalent Anti-fungal Disease Gene Mediated by Ri Plasmid

作　　者：王金杰[1] 王志英[1] 徐香玲[2] 张海玲[3] 

机构地区：[1]东北林业大学林学院,哈尔滨150040 [2]哈尔滨师范大学生命与环境科学学院,哈尔滨150025 [3]黑龙江省农业科学院草业研究所,哈尔滨150086

出　　处：《中国农学通报》2010年第6期35-38,共4页Chinese Agricultural Science Bulletin

基　　金：黑龙江省攻关课题"从碱草中克隆抗逆基因脱宽番茄种质资源的研究"(GA06B103-10);黑龙江省重大攻关项目"野生五味子驯化;栽培技术"(010-413684)

摘　　要：以番茄新品种T431、1911、0701为材料,以9天苗龄的叶片为受体,通过农杆菌Ri质粒介导,将几丁质酶基因和β-1,3葡聚糖酶基因转化番茄。共获得再生植株100株,经PCR技术检测,导入几丁质酶基因和β-1,3葡聚糖酶基因的植株分别为27株和13株,转化率为27%和13%;两个目的基因同时导入的植株10株,转化率为10%。PCR-Southern检测呈阳性,表明外源基因已经整合到番茄基因组中。New tomato varieties T431, 1911 and 0701 were used as the materials, chitinase gene and β -1, 3-glucanase gene were transformed into tomatoes by Agrobacterium-mediated transformation. A total of 100 regeneration plants were obtained. In these regenerated plants, 27 and 13 plants appeared respectively to be positive in PCR test with chitinase gene and β -1,3-glucanase gene primer. The transferring frequency of foreign gene was 27% and 13% separately. If the two genes were done simultaneously, 10 plants appeared to be positive in PCR test with a transferring frequency of 10%. PCR-Southern analysis of these plants showed that they produced positive hybridization, indicating that the chitinase gene and β -1,3-glucanase gene have been conveyed and integrated into genome of these plants.

关 键 词：番茄 发根农杆菌 几丁质酶基因 β-1 3葡聚糖酶基因 转化植株 

分 类 号：Q78[生物学—分子生物学]