闽引圆叶决明两个辐射变异新品系的若干特性研究  

Study on Agronomic Characters of Two Changed—Plants of Chamaecrista rotundifolia cv. MINYIN by Irradiation

作　　者：郑向丽[1] 叶花兰[1] 徐国忠[1] 

机构地区：[1]福建省农业科学院农业生态研究所福建山地草业工程技术研究中心,福州350013

出　　处：《中国农学通报》2010年第6期73-76,共4页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑计划项目"热带亚热带地区超高产牧草新品种选育及产业化示范"(2008BADB3B08);福建省星火计划项目"红壤丘陵开发地生态恢复与综合治理开发模式示范"(2007S007);福建省自然基金"山地红壤-豆科牧草(圆叶决明)系统中氮素迁移转化规律的研究"(D0610002)项目共同资助

摘　　要：利用60Coγ射线辐射闽引圆叶决明86134牧草种子,通过M1-M3代田间试验观察和筛选,筛选到两个86134的辐射变异后代,进行M4、M5代田间小区品比试验,结果表明:2个变异品系的茎叶比大幅度下降,其生物量分别增长39.26%和38.04%,达极显著水平,86134-32-3的粗脂肪含量极显著增长。RAPD分析表明,辐射后代与原种的DNA指纹图谱存在明显差异。The seeds of Chamaecrista rotundifolia cv.MINYIN pasturages were irradiated by 60Co-γ . Based on the observations of M1, M2, M3 generation in field experiment, we selected two plants with bigger variations in agronomic character to study in M4 and M5 generation. The results showed that the ratio of stem and leaf of two changed-plants decreased, The biomasses have increased 39.26% and 38.04% at 0.01 level. Crude fattiness of mutant 86134-32-3 increased at 0.01 level. On the basis of analysis by using random amplified polymorphic DNA （RAPD） markers, it was found that there were obvious differences in DNA fingerprinting between the progenies after irradiation by 60Co-γ and their parents.

关 键 词：闽引圆叶决明 辐射 诱变育种 

分 类 号：S3[农业科学—农艺学]