细菌性阴道病加特纳菌检测的临床价值  

Value of Detection of Gardnerella vaginalis in Diagnosis of Bacterial Vaginosis

作　　者：周湧[1] 李明[1] 王欲扬[1] 

机构地区：[1]广东省东莞市太平人民医院,广东东莞523900

出　　处：《实用预防医学》2010年第3期435-437,共3页Practical Preventive Medicine

基　　金：2006年东莞市科技计划项目

摘　　要：目的研究超高倍显微镜观察阴道分泌物查线索细胞、分离培养法、胺试验3种检测方法在细菌性阴道病(BV)加特纳菌检测中的临床价值,并进行阴道加德纳菌的药物敏感试验,以指导临床治疗。方法分别用超高倍显微镜观察阴道分泌物查线索细胞,分离培养法,胺试验3种检测方法对208例下生殖道感染患者行细菌性阴道病加德纳菌检测,并进行阴道加特纳菌的药物敏感试验。结果BV、非细菌性阴道病(NBV)患者均经线索细胞检查,BV试验和GV培养,BV患者组三种方法检测到的GV均高于NBV组(P<0.05);加特纳菌对万古霉素、菌必治、痢特灵敏感性较高。结论阴道分泌物直接涂片找加特纳菌和线索细胞、细菌分离培养、BV快速诊断等方法在细菌性阴道病(BV)加特纳菌检测中较PCR、免疫荧光法等简便易行。细菌培养及药物敏感试验,对复发性和久治不愈的BV治疗有重要的指导意义。Objective To evaluate the efficacy of identification of clue cells on vaginal smear,isolated cell culture and amine test with super microscope in detection of Gardnerella vaginalis in bacterial vaginosis,and to conduct the sensitivity test of Gardnerella vaginalis for guiding the clinical treatment. Methods Gardnerella vaginalis was examined in 208 patients with low reproductive tract infection by identification of clue cells on vaginal smear,isolated cell culture and amine test,and drug sensitivity test was performed for Gardnerella vaginalis. Results When identification of clue cells on vaginal smear,isolated cell culture and amine test were used,the diagnosis rates of Gardnerella vaginalis in patients with bacterial vaginosis were higher than those in non-bacterial vaginosis group （P0.05）. Gardnerella vaginalis was sensitive to vancomycin,ceftriaxone and furazolidone. Conclusions Identification of clue cells on vaginal smear,isolated cell culture and amine test are effective in diagnosis of bacterial vaginosis. Microbiological culture and drug sensitivity test are helpful for the treatment of recurrent and uncured bacterial vaginosis.

关 键 词：细菌性阴道病 加特纳菌 耐药性 检测 

分 类 号：R378[医药卫生—病原生物学]