草鸡J亚群禽白血病病毒的PCR检测  

PCR Method for Detecting J-subtype of Chicken Leukosis Virus in Local Chicken

作　　者：张敬峰[1] 张晓勇[2] 赵毓郎[3] 魏雪涛[1] 李银[1] 刘宇卓[1] 

机构地区：[1]江苏省农业科学院兽医研究所农业部动物疫病诊断与免疫重点开放实验室国家兽用生物制品工程技术研究中心,江苏南京210014 [2]浙江嘉兴出入境检验检疫局,浙江嘉兴314001 [3]苏州出入境检验检疫局外来有害生物防控实验室,江苏苏州215021

出　　处：《江西农业学报》2010年第3期5-7,共3页Acta Agriculturae Jiangxi

基　　金：江苏省农业自主创新资金项目[cx(08)142]

摘　　要：通过对GenBank发表的ALV的基因序列的分析,针对J型ALV保守区设计并合成1对特异性引物。通过对反应条件的优化,确定了检测ALV的PCR检测方法,对来源于江苏地区不同草鸡养殖基地的10份病料组织样本提取DNA进行PCR扩增。结果对疑似J亚群ALV病料和正常鸡肝脏组织的病原核酸检出率分别为90%和0,同时将扩增的目的基因进行克隆测序,并与GenBank中的参考毒株进行比较,结果表明目的基因片段序列长300 bp左右,与参考毒株核苷酸序列同源性为97%以上。试验表明,江苏地区草鸡群中确实存在J亚群鸡白血病病毒感染的情况。According to the conserved gene sequences of ALV in GenBank, a pair of the specific primers were designed. Through the optimization of reaction conditions, the PCR detection method was established. Then the ten tissue samples separated from different local chicken breeding bases in Jiangsu province were detected by PCR. The results showed that 90% of the suspected samples were ALV positive and the normal chicken liver tissue was ALV negative. The amplified target gene was cloned, sequenced and compared with the reference nucleotide sequence in GenBank, the results showed that the target gene fragment was 300 bp, and the homology of nucleotides was more than 97%. The study showed that J - subtype ALV existed in the local chickens in Jiangsu province.

关 键 词：鸡白血病病毒 PCR 检测 

分 类 号：S851.347.2[农业科学—预防兽医学]