RNA干扰抑制COX-2的重组腺病毒的构建及体外抗肿瘤活性  

Construction of Adenovirus Armed with shRNA(COX-2) and Investigation of Its Anti-tumor Activity in Vitro

作　　者：陈青[1] 娄文加[1] 钱程[1] 刘立[1] 

机构地区：[1]浙江理工大学生命科学院新元医学与生物技术研究所,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2010年第2期292-296,共5页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：国家重点基础研究发展计划基金项目(973)(2004CB518804);高科技发展基金项目(863)(2006AA02Z126);浙江省重中之重学科开放基金资助(SWYX0816)

摘　　要：环氧化酶2(cyclooxygenase-2,COX-2)是肿瘤治疗中的一个重要的靶向基因。可COX-2抑制剂因易引发肝损伤的副作用,影响了COX-2抑制剂在临床上的应用。本研究构建出运载小干扰RNA的重组腺病毒试图解决上述难题。实验结果显示:癌细胞系中的COX-2成功被抑制,同时活细胞计数实验结果表明COX-2在癌细胞中的抑制能促使癌细胞生长受到抑制,而正常细胞不受到影响,证明该载体系统能有效解决COX-2抑制剂易引发肝损伤的毒副作用,为其临床应用提供了坚实的基础。Cyclooxygenase-2 （COX-2） is an important target gene in cancer therapy. However, COX-2 inhibitors generally induce liver injury and it is controvertible to applied COX-2 inhibitors in clinic therapy. In order to overcome the above obstacle, the authors construct an adenovirus armed with a small interfer- ence RNA. Our results show that COX-2 in cancer cell lines is successfully inhibited. Meanwhile, MTT result suggests that silence of COX-2 can inhibit the growth of cancer cells without any damage to normal cells. It is indicated that the adenovirus system can effectively avoid the liver toxicity induced by COX-2 in- hibitor, providing a solid foundation for application in clinical therapy.

关 键 词：RNA干扰 环氧化酶-2 腺病毒 

分 类 号：Q789[生物学—分子生物学]