反相高效液相色谱法测定壳糖止血海绵中诺氟沙星和盐酸利多卡因的含量  

Determination of lidocaine hydrochloride and norfloxacine in chitosan hemostatic sponge by RP-HPLC

作　　者：王曙东[1] 潘璟[1] 方李[1] 夏曙辉[1] 宋炳生[1] 

机构地区：[1]南京军区南京总医院制剂科,江苏南京210002

出　　处：《药学实践杂志》2010年第1期45-47,共3页Journal of Pharmaceutical Practice

基　　金：南京市科技发展计划资助项目(20028006)

摘　　要：目的建立反相高效液相色谱法测定壳糖止血海绵中诺氟沙星和盐酸利多卡因含量的测定方法。方法采用Hedera—ODS-3 C18色谱柱（4．6mm×250mm，5μm），以乙腈-1％磷酸溶液（12：88）为流动相，三乙胺调节pH3．0±0．1，检测波长为254nm。结果诺氟沙星和盐酸利多卡因在0．025—0．100mg／ml浓度范围内均有良好线性关系。诺氟沙星平均回收率为98．84％，RSD为1．72％，盐酸利多卡因平均回收率为100．24％，RSD为1．86％。结论该法简便准确，稳定无干扰，重现性好，可用作壳糖止血海绵中诺氟沙星和盐酸利多卡因的含量测定。Objective To establish a quantitative determination method for lidocaine hydrochloride and norfloxacine in chitosan hemostatic sponge by RP-HPLC. Methods The determination was carried out with a Hedera-ODS-3 C18 column （4.6 mm × 250 mm, 5 μm）. The mobile phase consisted of acetonitrile-1% phosphoric acid solution （12 ： 88 ） , with pH adjusted to 3.0 ±0.1 by using triethylamine. Detecting wavelength was at 254 nm. Results The linear range for lidocaine hydrochloride and norfloxacine was both 0. 025 - 0. 100 mg/ml. The average recovery and RSD of norfloxacine were 98.84% and 1.72% , and those of lidocaine hydrochloride were 100.24% and 1.86%. Conclusion The method offers simple instrumental operation, good stability, and high accuracy for the determination of the chitosan hemostatic sponge.

关 键 词：壳糖止血海绵 诺氟沙星 盐酸利多卡因 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]