子痫前期产妇胎盘床HIF-1α蛋白的表达与母体外周血vWF浓度的变化  

Change of HIF-1α protein expression in the placenta bed and concentration of vWF in maternal peripheral blood of pre-eclampsia

作　　者：彭梅[1] 丁依玲[1] 喻玲[1] 周昌菊[2] 

机构地区：[1]中南大学湘雅二医院妇产科,长沙410011 [2]中南大学湘雅三医院妇产科,长沙410013

出　　处：《中南大学学报（医学版）》2010年第2期134-139,共6页Journal of Central South University ：Medical Science

摘　　要：目的:研究子痫前期产妇胎盘床低氧诱导因子-1α(hypoxia-inducible factor1α,HIF-1α)蛋白的表达与母体外周血血管性假血友病性因子(von Willbrand Factor,vWF)浓度的变化,并探讨它们在子痫前期发病机制中的作用。方法:选取正常妊娠产妇、子痫前期轻度及子痫前期重度产妇各20例,采用Western印迹方法检测胎盘床HIF-1α蛋白的表达,酶联免疫吸附法(ELISA)检测母体外周血vWF的浓度;Spearman相关分析比较两者的相关性。结果:HIF-1α蛋白在子痫前期重度组胎盘床中的表达最高,其次是子痫前期轻度组,对照组最低,组间比较差异均有统计学意义(均P<0.001)。vWF浓度在子痫前期重度组母体外周血中最高,其次是子痫前期轻度组,对照组最低,组间比较差异均有统计学意义(均P<0.05)。胎盘床HIF-1α蛋白的表达与母体外周血vWF浓度呈正相关(r1=0.65,P<0.001);母体外周血vWF浓度与子痫前期的病情程度呈正相关(r2=0.61,P<0.001)。结论:HIF-1α和vWF可能在子痫前期的发生和发展中起协同作用。Objective To investigate the expression of HIF-1α protein in the placenta bed and the concentration of yon Willrand factor （ vWF ） in maternal peripheral blood from pre-eclampsia and normal pregnancy, and to determine the effect of HIF-1α and vWF on the pathogenesis of preeclampsia. Methods Forty pre-eclampsia patients （20 mild and 20 severe ） were recruited as 2 study groups, and another 20 normal pregnant women were served as a normal group. Western blot was used to detect the expression of HIF-1α protein in the placenta bed. ELISA was adopted to detect the concentration of vWF in the maternal peripheral blood. Correlation between HIF-1 α protein expression and vWF level was analyzed by Spearman method. Results The expression of HIF-1α protein in the placenta bed was the highest in the severe pre-eclampsia patients among 3 groups, followed by the mild pre-eelampsia patients and the normal controls. There was significant difference among 3 groups （ P 〈 0.001 ）. The concentration of vWF in the maternal peripheral blood was the highest in the severe,pre-eclampsia patients, followed by the mild pre-eclampsia patients and the normal controls. There was significant difference among the 3 groups （ P 〈 0. 05 ）. Postive correlation was found between the expression of HIF-1 α protein in the placenta bed and the concentration of vWF in the maternal peripheral blood in patients with pre-eclampsia （ r1 = 0. 65, P 〈 0. 001 ） , and between the concentration of vWF in the maternal peripheral blood and the pathogenetic condition degree of pre-eelampsia （ r2 =0. 61, P 〈 0. 001 ）. Conclusion HIF-1α in coordination with vWF may play an important role in the pathogenesis of pre-eclampsia.

关 键 词：子痫前期 胎盘床 低氧诱导因子1Α 血管性假血友病性因子 

分 类 号：R714.245[医药卫生—妇产科学]