一株DBP高效降解菌的筛选及其降解特性  

Isolation and characterization of a highly efficient DBP-degrading bacterium

作　　者：金德才[1] 梁任星[1] 王洋洋[1] 代沁芸[1] 张瑞永[1] 吴学玲[1] 

机构地区：[1]中南大学资源加工与生物工程学院,湖南长沙410083

出　　处：《中南大学学报（自然科学版）》2010年第1期8-14,共7页Journal of Central South University:Science and Technology

基　　金：国家自然科学基金资助项目(30770388)

摘　　要：从污染土壤中筛选到一株能够以酞酸酯(Phthalic acid esters,PAEs)为唯一碳源和能源生长的微生物,命名为JDC-11,对其进行鉴定和降解特性研究,并考查不同葡萄糖浓度对其降解的影响。采用形态学、生理生化和16SrDNA序列分析进行鉴定。采用高效液相色谱(HPLC)测定菌株JDC-11在摇瓶中对邻苯二甲酸二丁酯(DBP)的降解能力。研究结果表明:该菌株为Rhodococcussp.;最佳降解条件是温度为30℃,初始pH值为8.0,转速为175r/min,在此最佳条件下,JDC-11能够在24h内将1g/LDBP完全降解,证明JDC-11是一株高效降解菌。在降解过程中的前12h,葡萄糖的存在均抑制DBP的降解,12h后,200mg/L的葡萄糖仍抑制降解,而质量浓度≥400mg/L的葡萄糖加速了DBP的降解效率。A bacterial strain which could grow well on the medium with the substrate of PAEs as the sole source of carbon and energy was isolated from contaminated soil and it was designated as JDC-11. Then the bacterium was identified and its degrading capability was studied. Effects of different glucose concentrations on DBP degradation were also tested. The strain JDC-11 was identified based on 16S rDNA sequence analysis and its morphological and physiological characteristics. The biodegradation experiments of DBP were performed in shake flasks and its DBP degradation capability was measured by high performance liquid chromatography. The results show that strain JDC-11 is identified as Rhodococcus sp. The optimum conditions for DBP degradation by strain JDC-11 are as follows： initial pH is 8.0, the temperature is 30 ℃ and agitation rate is 175 r/min. After 24 h, 1 g/L of DBP is degraded completely under the optimum conditions. It is demonstrated that JDC-11 is a highly efficient strain in degrading DBP. All concentration of glucose inhibits the degradation of DBP in the initial 12 h. Furthermore, when the glucose concentration is 200 mg/L, degradation of DBP is also inhibited in the latter 12 h. Meanwhile, the concentration of glucose above 400 mg/L（including 400 mg/L） will increase the DBP degradation rate.

关 键 词：邻苯二甲酸二丁酯 16SrDNA 生物降解 HPLC 

分 类 号：TQ414.1[化学工程]