中度嗜热混合菌在搅拌槽中浸出黄铜矿及其群落动态  

Bioleaching of chalcopyrite by moderately thermophilic mixed microorganisms in stirred tank bioreactor and community succession analysis

作　　者：周洪波[1] 谢英剑[1] 张汝兵[1] 曾伟民[1] 罗海浪[1] 王玉光[1] 

机构地区：[1]中南大学资源加工与生物工程学院,生物冶金教育部重点实验室,湖南长沙410083

出　　处：《中南大学学报（自然科学版）》2010年第1期15-20,共6页Journal of Central South University:Science and Technology

基　　金：国际海底区域研究开发“十一五”项目(DYXM-115-02-2-07);海洋公益性行业科研专项经费资助项目(200805032);教育部新世纪优秀人才支持计划项目(NECT-06-0691)

摘　　要：为了优化浸出工艺,研究浸矿体系中主要微生物种群的结构和动态变化,在槽式搅拌反应器中于45℃用中度嗜热混合菌(Acidithiobacillus caldus,Leptospirillum ferriphilum,Sulfobacillus acidophilus,Sulfobacillus thermosulfidooxidans等)浸出黄铜矿精矿,考察浸出液的pH,电极电位Eh及金属离子浓度并应用PCR-RFLP(限制性酶切片段长度多态性)方法研究细菌群落变化,浸出时间为30d。研究结果表明:Cu的浸出率为26.2%,反应器内菌种多样性不丰富,只有At.caldus和L.ferriphilum2种;群落动态变化明显,在浸出开始阶段At.caldus是优势种群,其丰度为96%,随着浸出的进行,L.ferriphilum逐渐增多,在浸出后期代替At.caldus成为优势种群,其丰度为69%。Community succession in bioleaching process was investigated for optimization. Chalcopyrite concentrate was leached by moderately thermophilic mixed microorganisms, including Acidithiobacillus caldus, Leptospirillum ferriphilum, Sulfobacillus acidophilus, Sulfobacillus thermosulfidooxidans et al. in a stirred tank bioreactor operated at 45 ℃ for 30 d. The parameters such as pH, Eh and concentrations of metal ions were determined and synchronously the method of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism （PCR-RFLP） was used to analyze the succession of bacterial community, and their possible influences on succession of community were discussed. The results show that copper recovery is 26.2%. Only two species, i.e., At. caldus and L. ferriphilum are detected. At the beginning of bioleaching process, At. caldus is the predominant species, accounting for 96% of the whole community. During the process of leaching, L. ferriphilum gradually increases and replaces At. caldus, and accounts for 69% as the majority of community in the later stage of bioleaching process.

关 键 词：黄铜矿精矿 槽式搅拌反应器 中度嗜热菌 PCR-RFLP 群落变化 

分 类 号：TF18[冶金工程—冶金物理化学] Q939[生物学—微生物学]