中国朝鲜族DXS7132、DXS6854和GATA31E08基因座遗传多态性  

Genetic Polymorphisms of DXS7132,DXS6854,and GATA31E08 STR Loci Found in a Korean Ethnic Group in China

作　　者：张永吉[1] 徐京男 张美花[1] 韩松瑛[1] 

机构地区：[1]延边大学基础医学院法医学教研室,延吉133000 [2]延边州公安局刑事科学技术研究所,延吉133000

出　　处：《人类学学报》2010年第1期95-99,共5页Acta Anthropologica Sinica

基　　金：国家自然科学基金(30660201)

摘　　要：利用复合PCR扩增、变性聚丙烯酰胺凝胶电泳和银染方法对261名中国朝鲜族人群3个X-STR基因座研究发现，在DXS7132、DX6854和GATA31E08中分别检出8个、6个和10个等位基因；基因频率分别分布在0．006～0．344、0．015～0．450和0．005～0．317之间；分别检出19、17和21种基因型，女性个体基因型频率分布均符合Hardy—Weinberg平衡（P〉0．05）；观测杂合度分别为0．706，0．743，0．772；多态信息含量分别为0．710，0．730，0．670；女性个人识别力分别为0．898，0．911，0，857；男性个人识别力分别为0．766，0．802，0．734；联体单倍型多样性大于0．988。结果表明，3个基因座均具有较高的杂合度、多态信息量和个人识别力，是较理想的遗传标记系统，在研究中国朝鲜族起源和与其他民族之间的亲缘关系等方面具有较高的应用价值，并且对中国朝鲜族群体的亲子鉴定和个人识别提供宝贵的基础资料。The purpose of this research was to understand the allele structure and genetic polymorphism at DXS7132, DXS6854, and GATA31E08 short tandem repeats （STRs） loci in a Korean ethnic group found in the Jilin Yanbian area, and to construct a preliminary descriptive database. The allele frequencies of the three STRs loci in 261 unrelated individuals from this ethnic group were analyzed by multiplex polymerase chain reaction （PCR） and polyacrylamide gel eleetrophoresis （PAGE）. The following 8, 6 and 10 alleles and 19, 17 and 21 genotypes were observed at the DXS7132, DXS6854 and GATA31E08 STR loci, respectively. All of these loci （in females） met the Hardy-Weinberg equilibrium （P 〉 0.05 ）. Statistical analysis of the three STR loci calculated the heterozygosities at 0. 706, 0. 743, and 0. 772; the polymorphic information contents （PIC） at 0. 710, 0. 730 and 0.67. The female powers of discrimination （PD） were 0.898, 0.911 and 0.857. The reals genetic diversity of the male samples were 0. 766, 0. 802 and 0. 734, and the combined haplotype diversity was more than 0. 988. All of these results show that all three of the loci in this study were found to have high heterozygosity and polymorphic information content, and thus, they could provide useful genetic markers. These results could serve as valuable data to enrich the Korean ethnic group genetic database and play an important role in application and understanding of Chinese population genetics.

关 键 词：朝鲜族 短串联重复序列 DXS7132 DX6854 GATA31E08 

分 类 号：Q987[生物学—遗传学]