高糖环境对肾系膜细胞NF—κB／IκB通路的影响及其调控机制  

Effect of high glucose environment on the NF-κB/IκB signal pathway in cultured rat glomerular mesangial cell and its mechanism

作　　者：徐玲[1] 李佳[1] 蒋岚[1] 徐勇[1] 钟海花[1] 

机构地区：[1]泸州医学院附属医院内分泌科,646000

出　　处：《中华内分泌代谢杂志》2010年第2期152-154,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：基金项目：国家自然科学基金资助项目（30670980）;四川省杰出青年基金资助项目（08ZQ026-366）

摘　　要：探讨高糖对肾系膜细胞IκB-αmRNA和IκB-α、NF—κBp65蛋白表达和NF—κBp65核转位的影响及作用途径。结果发现高糖促进IκB—α蛋白降解和NF—κBp65核转位，特异性泛素一蛋白酶体阻断剂MG132可明显逆转高糖导致的IKB-α蛋白降解和NF-κBp65核转位，提示高糖可通过泛素一蛋白酶体途径导致NF—κB信号通路的活化。To explore the effect of high glucose on the NF-κB/IκB signal pathway in cultured rat glomeru]ar mesangial cells. The results showed that high glucose increased the degradation of IκB-α and the translocation to nucleus of NF-κB. These changes could be reverted mostly by MG132, a proteasome inhibitor. It suggests that the activation of the NF-κB signal pathway by high glucose concentration may probably be via the ubiquitin-proteasome pathway.

关 键 词：糖尿病肾病 高糖 NF—κB MG132 泛素-蛋白酶体途径 

分 类 号：R587.2[医药卫生—内分泌]