利用排泄行为参数评价重金属暴露对于秀丽线虫代谢状态的毒害(英文)  

Evaluation of the metabolism state by endpoint of defecation behavior in metal exposed nematode Caenorhabditis elegans

作　　者：武秋立[1] 杨燚超[1] 王大勇[1] 

机构地区：[1]东南大学医学院生物化学与分子生物学系,发育与疾病相关基因教育部重点实验室,江苏南京210009

出　　处：《东南大学学报（医学版）》2010年第1期23-29,共7页Journal of Southeast University(Medical Science Edition)

基　　金：supported by the grants from the National Natural Science Foundation of China(No.30870810);the Program for New Century Excellent Talents in University

摘　　要：目的:研究重金属暴露对秀丽线虫排泄行为的影响。方法:用于分析的CdCl2、CrCl2、HgCl2和Pb(NO3)2溶液浓度分别为50、100、150和200μmol.L-1。参数平均排泄循环长度用于评价重金属暴露秀丽线虫的代谢状态改变。线性回归用于分析平均排泄循环长度与暴露重金属浓度之间、暴露动物中平均排泄循环长度和咽泵运动速率之间的关联。结果:所有分析浓度的汞和铅暴露以及浓度为100μmol.L-1到200μmol.L-1的镉和铬暴露均可以显著增加线虫的平均排泄循环长度。线虫平均排泄循环长度具有伴随重金属暴露浓度改变而变化的特点,且平均排泄循环长度显著相关于所暴露重金属的浓度。在重金属暴露的线虫中,咽泵运动速率还显著相关于平均排泄循环长度。此外,汞和铅暴露对于线虫排泄行为的影响远比铬和镉的暴露严重。结论:参数平均排泄循环长度可以有效评价重金属暴露对线虫代谢状态的毒害,而且在重金属暴露线虫中可以观察到平均排泄循环长度与咽泵运动速率之间的显著相关性。Objective：To investigate the effects of metal exposure on defecation behavior in nematode Caenorhabditis elegans.Method：The concentrations of CdCl2,CrCl2,HgCl2,and Pb（NO3）2 metal solutions used were 50 μmol·L-1,100 μmol·L-1,150 μmol·L-1,and 200 μmol·L-1,respectively.The endpoint of mean defecation cycle length was used to evaluate the metabolism state in metal exposed nematodes.The linear regression analysis was performed to investigate the possible associations for the concentration of exposed metals with mean defecation cycle length,and between pumping rate and mean defecation cycle length.Results：Exposure to Hg and Pb at all concentrations and exposure to Cr and Cd at concentrations from 100 μmol·L-1 to 200 μmol·L-1 induced a significant increase of mean defecation cycle length.Alterations of mean defecation cycle length in metal exposed nematodes exhibited a concentration-dependent manner.In addition,mean defecation cycle length was significantly correlated with the concentrations of exposed metals tested.Moreover,the close association between pumping rate and mean defecation cycle length could be formed in metal exposed nematodes.Furthermore,the toxicity on defecation behavior from exposure to Hg and Pb was more severe than that from exposure to Cr and Cd in nematodes.Conclusion：The endpoint of mean defecation cycle length can be used to effectively evaluate the toxicity on metabolism state from metal exposure,and the close association of mean defecation cycle length with pumping rate can be observed in metal exposed nematodes.

关 键 词：重金属 排泄行为 代谢状态 秀丽线虫 

分 类 号：R114[医药卫生—卫生毒理学]