热激胁迫可能通过降低脂肪与糖原的积累抑制涡虫的发育(英文)  

Heat-shock stress suppresses the development possibly by decreasing lipid and glycogen storage in planarian Dugesia japonica

作　　者：刘培党[1] 林兴凤[1] 王大勇[1] 

机构地区：[1]东南大学医学院生物化学与分子生物学系,发育与疾病相关基因教育部重点实验室,江苏南京210009

出　　处：《东南大学学报（医学版）》2010年第1期36-41,共6页Journal of Southeast University(Medical Science Edition)

基　　金：supported by the grants from the National Natural Science Foundation of China(No.30870810);the Program for New Cen-tury Excellent Talents in University

摘　　要：目的:探讨热激胁迫对于涡虫发育以及脂肪和糖原积累的影响。方法:涡虫的发育通过体长与体质量来评价。分析35℃热激胁迫处理涡虫的体长与体质量变化以及实质细胞中脂肪与糖原积累情况,并评价热激胁迫处理涡虫中发育与脂肪或糖原积累之间可能的相关性。结果:与未经历热激处理的对照相比,热激胁迫处理不同长度时间(16、20、24、28与32 h)后涡虫体长与体质量显著减少或降低。与对照相比,热激胁迫处理导致涡虫实质细胞中的脂肪颗粒数量明显降低、尺寸显著变小。类似地,热激胁迫处理还导致涡虫实质细胞中的糖原积累的显著降低。进而,线性回归分析表明热激胁迫处理涡虫的体长与体质量均显著相关于实质细胞中脂肪颗粒的尺寸或糖原的含量。结论:热激胁迫处理可以明显抑制涡虫的发育,且热激胁迫处理涡虫中存在发育与脂肪或糖原积累之间的显著关联。Objective：To investigate the effects of heat-shock on development and accumulation of lipid and glycogen in planarian Dugesia japonica.Methods：The development of planarians was evaluated by the body length and body weight.The body length,body weight,and the accumulation of lipid and glycogen in parenchymal cells were examined in heat-shock（35 ℃） treated planarians.The possible associations of development with lipid or glycogen storage were further analyzed in heat-shock treated planarians.Results：After heat-shock treatment for different time intervals（16,20,24,28,and 32h）,the body weights and body lengths of heat-shock treated planarians were all significantly decreased compared with those in control planarians without heat-shock treatment.Compared with the lipid accumulation in control planarians,heat-shock treatment resulted in the sharp decrease of lipid droplet number and the reduction of lipid droplet size in parenchymal cells.Similarly,the relative intensities of labeled signals for glycogen in parenchymal cells were significantly decreased after heat-shock treatment.Moreover,linear regression analysis suggests that both the body weight and the body length were significantly correlated with the relative size of lipid droplet and the relative intensity of glycogen signal in heat-shock treated planarians.Conclusion：Treatment with the stress of heat-shock can obviously suppress the development of planarians,and the close association between the development and the accumulation of lipid or glycogen exists in heat-shock treated planarians.

关 键 词：热激胁迫 脂肪 糖原 发育 涡虫 

分 类 号：R114[医药卫生—卫生毒理学]