PPAR-γ配体对特发性血小板减少性紫癜模型小鼠NO生成诱导时间效应的影响  

Study on time effect of NO production induced by PPAR-γ ligand in ITP model mice

作　　者：于利霞 于利凌[2] 金呈强[2] 刘仿[3] 

机构地区：[1]山东省贸易职工医院,山东济宁272000 [2]济宁医学院附属医院,山东济宁272029 [3]广东医学院微生物学与免疫学教研室,广东湛江524023

出　　处：《济宁医学院学报》2010年第1期4-7,共4页Journal of Jining Medical University

基　　金：广东省自然科学基金(NO:06028959);广东省东莞市科技计划项目(NO:2008108101033);广东医学院青年基金(NO:XQ06017)

摘　　要：目的探讨ITP模型小鼠体内,过氧化物酶体增生物激活受体γ(PPAR-γ)对一氧化氮(NO)生成的影响及意义。方法90例ITP模型小鼠随机分成罗格列酮试验组、正常对照组和磷酸盐缓冲液(PBS)对照组,每组30例。罗格列酮试验组用20μmol/L的罗格列酮进行灌胃。硝酸还原酶法检测小鼠血液NO含量,RT-PCR法检测血液淋巴细胞PPAR-γmRNA表达情况。结果血清中NO在第6h,12h,18h,24h和30h的含量罗格列酮试验组高于正常对照组及PBS对照组(均P<0.05)。在罗格列酮试验组中,NO含量及PPAR-γmRNA的表达随着用药时间的延长而增加(均P<0.05),NO表达量与PPAR-γ的表达呈正相关(r=0.89,P<0.05)。结论在ITP小鼠模型内,PPAR-γ活化剂能够以时间依赖的形式增加NO的生成,PPAR-γ可能通过NO途径来发挥相应的生理功能。Objective To investigate the influence and significance on nitric oxide （NO） production caused by PPAR-γ, in ITP model mice. Methods Ninety ITP model mice were divided into rosiglitazone experimental group, normal control group and phosphate buffered saline （PBS） control group with 30 in each group,rosiglitazone experimental group with the same concentration （20μmol/L） of the PPAR-γ activator Thiazolidine drug rosiglitazone for enema,and we detected NO content by nitrate reductase in blood of the mice, and tested the expression of PPAR-γ mRNA in blood Iymphocytes by RT--PCR. Results Rosiglitazone experimental group was higher than the normal control group and PBS control group in the form of serum NO content in the first 6h, 12h, 18h, 24h and 30h （all P〈0.05 ）. In rosiglitazone experimental group, NO concentration increased with the duration of drug usage, NO content had significant differences at the measurement time points （P〈0. 05）, PPAR-γmRNA expression increased with the time （ P〈0. 05）, NO content and the expression of PPAR-γ, expression were posi- tively correlated （r=0. 89,P〈0.05）.Conclusion PPAR-γ, activator was able to increase NO production relying on the time of drug usage and PPAR-γ might adjust the target factors by NO pathway in ITP model mice.

关 键 词：一氧化氮 一氧化氮合酶 特发性血小板减少性紫癜 PPAR-Γ 

分 类 号：R3[医药卫生—基础医学]