葱蝇的实验室饲养、生物学特性及滞育诱导  

Laboratory Rearing,Biological Characteristics and Diapause Induction of the Onion Maggot,Delia antiqua

作　　者：陈斌[1] 黎万顺[1] 冯国忠[1] 何正波[1] 李廷景[1] 

机构地区：[1]重庆师范大学生命科学学院昆虫与分子生物学研究所重庆高校生物活性物质工程研究中心重庆高校动物生物学重点实验室,重庆400047

出　　处：《重庆师范大学学报（自然科学版）》2010年第2期9-13,共5页Journal of Chongqing Normal University:Natural Science

基　　金：国家自然科学基金(No.30870340);重庆市自然科学基金重点项目(No.CSTC2008BA5030);重庆市自然科学基金(No.CSTC2008BB1365)

摘　　要：葱蝇是昆虫滞育分子机理、滞育期抗逆基因、冬滞育和夏滞育专化基因比较等研究的理想模式种,为开展昆虫滞育分子机理及抗逆基因研究,从日本引进葱蝇并设计了葱蝇的培养笼,首次在国内建立了稳定的实验室种群。基于早前的研究工作基础,对葱蝇最佳食物、人工饲养技术和条件、冬滞育和夏滞育诱导和终止条件作了进一步研究;并对各虫期、冬滞育和夏滞育期的生理和生物学特性作了进一步观察;成功地获得了供分子生物学研究需要的非滞育、冬滞育和夏滞育葱蝇各发育阶段的样品。特别重要的是,在本研究通过适度提高光照强度可以使所有实验蛹进入夏滞育。因此,本实验室能够获得100%的冬滞育和夏滞育蛹。本文报道和总结葱蝇的实验室饲养技术、各虫态主要生物学习性,以及冬滞育和夏滞育的诱导和终止策略。The onion maggot, Delia antiqua, is an ideal model species for the studies of the molecular mechanism of insect diapause, the stress resistance genes during diapauses, and the comparison of winter- and summer-diapause-specific genes. To meet the need of the research of the molecular mechanism of insect diapause and the stress resistance genes during diapauses, we introduced the onion maggot from Japan, designed rearing cage for the species, and established a stable laboratory population for the first time in China. We optimized the food recipe for larvae, the technique and condition of artificial rearing, and the diapause induction and termination of both winter and summer diapauses of the onion maggot based on earlier publications. We further observed the physiological and biological characteristics in each stages of normal development, winter and summer diapauses, and obtained all stages of samples of non-diapausing, winter-diapausing and smnmer-diapausing pupae for the study of molecular biology. More importantly, we worked out the optima induce condition for summer-diapausing pupae to enhance the intensity of illumination, and acquired the pupae of 100% entering summer-diapause and winter-diapause. Here we report and review the laboratory rearing techniques, main biological characteristics, and the strategy of winter and summer diapause induction and termination.

关 键 词：葱蝇 实验室饲养 人工饲料 生物学特性 滞育诱导 

分 类 号：Q969.454.1[生物学—昆虫学] S433.89[农业科学—农业昆虫与害虫防治]